Specificity and sites of ETA receptor KO. Panel A shows a representative blot (N = 6 each gender) of PCR for ENDRA gene recombination, demonstrating Cre activity in liver and kidney. Panel B shows a representative blot (N = 8) of PCR for ENDRA gene recombination in microdissected thick ascending limb (TAL), proximal tubule (PT), cortical collecting duct (CCD) and glomeruli from Pax8-rtTA/LC1/floxed ETA (iETA) mice treated with doxycycline (DOX) (separate mice shown in each lane). Negative (homozygous floxed ETA (control)) and positive (iETA + DOX) PCR of whole kidney DNA are shown. Panel C shows real-time PCR of ETA mRNA (normalized to GAPDH mRNA) in cortex and inner medulla from control + DOX and iETA + DOX mice (N = 8 each group). *p < 0.05 vs. control + DOX.
Stuart et al. BMC Nephrology 2012 13:166 doi:10.1186/1471-2369-13-166