Schema of conditional KO of the ENDRA gene. The Pax8 promoter drives expression of rtTA which requires doxycycline to activate the bicistronic Cre recombinase and luciferase-expressing transgene. Cre is expressed specifically in the nephron but not in glomeruli. ETA receptor KO mice are homozygous for the floxed ENDRA gene and heterozygous for the two transgenes.
Stuart et al. BMC Nephrology 2012 13:166 doi:10.1186/1471-2369-13-166