Additional file 3.

Western analysis of wild-type and Mut embryonic fibroblast extracts. Western blotting using anti-mutase antisera reveals a band of ~80 kd in the wild-type extracts that is completely absent from the Mut null MEF line. Recombinant murine methylmalonyl-CoA mutase expressed in yeast served as a positive control (Y) and is located next to the marker (M) lane. The sizes of the molecular weight standards in kilodaltons are indicated. Anti-actin antibodies were used to control for the amount of protein loaded per well.

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Chandler et al. BMC Medical Genetics 2007 8:64   doi:10.1186/1471-2350-8-64