Open Access Highly Accessed Research article

Matrix Metalloproteinase-9 (MMP-9) polymorphisms in patients with cutaneous malignant melanoma

Javier Cotignola1, Boris Reva1, Nandita Mitra2, Nicole Ishill1, Shaokun Chuai1, Ami Patel1, Shivang Shah1, Gretchen Vanderbeek1, Daniel Coit1, Klaus Busam1, Allan Halpern1, Alan Houghton1, Chris Sander1, Marianne Berwick3 and Irene Orlow1*

Author Affiliations

1 Memorial Sloan-Kettering Cancer Center, New York, NY, USA

2 University of Pennsylvania, Philadelphia, PA, USA

3 University of New Mexico, Albuquerque, NM, USA

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BMC Medical Genetics 2007, 8:10  doi:10.1186/1471-2350-8-10

Published: 8 March 2007

Abstract

Background

Cutaneous Malignant Melanoma causes over 75% of skin cancer-related deaths, and it is clear that many factors may contribute to the outcome. Matrix Metalloproteinases (MMPs) play an important role in the degradation and remodeling of the extracellular matrix and basement membrane that, in turn, modulate cell division, migration and angiogenesis. Some polymorphisms are known to influence gene expression, protein activity, stability, and interactions, and they were shown to be associated with certain tumor phenotypes and cancer risk.

Methods

We tested seven polymorphisms within the MMP-9 gene in 1002 patients with melanoma in order to evaluate germline genetic variants and their association with progression and known risk factors of melanoma. The polymorphisms were selected based on previously published reports and their known or potential functional relevance using in-silico methods. Germline DNA was then genotyped using pyrosequencing, melting temperature profiles, heteroduplex analysis, and fragment size analysis.

Results

We found that reference alleles were present in higher frequency in patients who tend to sunburn, have family history of melanoma, higher melanoma stage, intransit metastasis and desmoplastic melanomas among others. However, after adjustment for age, sex, phenotypic index, moles, and freckles only Q279R, P574R and R668Q had significant associations with intransit metastasis, propensity to tan/sunburn and primary melanoma site.

Conclusion

This study does not provide strong evidence for further investigation into the role of the MMP-9 SNPs in melanoma progression.