G1P3 gene analyzed by QRT-PCR. Glucose-6-phosphate dehydrogenase (G6PDH) was used as a reference gene in relative quantification. The expression of G1P3 was on average 3.6 times higher in MS twins compared to their healthy siblings, and the results concurred with those obtained from cDNA microarray. Panel a shows the real-time QRT-PCR amplifications duplicate in each twin pair (3 – 6) and the table (panel b) shows the comparison between the cDNA microarray results and the QRT-PCR results. The y axis indicates fluorescence intensity and x axis PCR cycle numbers. G1P3 gene amplification begins earlier in MS patients than in their healthy siblings, indicating higher gene expression in MS twins.
Särkijärvi et al. BMC Medical Genetics 2006 7:11 doi:10.1186/1471-2350-7-11