Research article

Genetic analysis of the GLUT10 glucose transporter (SLC2A10) polymorphisms in Caucasian American type 2 diabetes

Jennifer L Bento^1,5 , Donald W Bowden^1,2,5 , Josyf C Mychaleckyj^2,3,4,5 , Shohei Hirakawa¹ , Stephen S Rich⁴ , Barry I Freedman² and Fernando Segade²

¹Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157, USA

²Department of Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157, USA

³Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157, USA

⁴Department of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157, USA

⁵Center for Human Genomics, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157, USA

author email corresponding author email

BMC Medical Genetics 2005, 6:42doi:10.1186/1471-2350-6-42

Published:	7 December 2005

Abstract

Background

GLUT10 (gene symbol SLC2A10) is a facilitative glucose transporter within the type 2 diabetes (T2DM)-linked region on chromosome 20q12-13.1. Therefore, we evaluated GLUT10 as a positional candidate gene for T2DM in Caucasian Americans.

Methods

Twenty SNPs including 4 coding, 10 intronic and 6 5' and 3' to the coding sequence were genotyped across a 100 kb region containing the SLC2A10 gene in DNAs from 300 T2DM cases and 310 controls using the Sequenom MassArray Genotyping System. Allelic association was evaluated, and linkage disequilibrium (LD) and haplotype structure of SLC2A10 were also determined to assess whether any specific haplotypes were associated with T2DM.

Results

Of these variants, fifteen had heterozygosities greater than 0.80 and were analyzed further for association with T2DM. No evidence of significant association was observed for any variant with T2DM (all P ≥ 0.05), including Ala206Thr (rs2235491) which was previously reported to be associated with fasting insulin. Linkage disequilibrium analysis suggests that the SLC2A10 gene is contained in a single haplotype block of 14 kb. Haplotype association analysis with T2DM did not reveal any significant differences between haplotype frequencies in T2DM cases and controls.

Conclusion

From our findings, we can conclude that sequence variants in or near GLUT10 are unlikely to contribute significantly to T2DM in Caucasian Americans.