MiR-663 is inactivated by promoter hypermethylation in AML cell lines. (A) MSP analysis of the methylation status of miR-663 in leukemia cells lines 72 h after treatment with 5-Aza, or the same volume of DMSO solvent control, shows that miR-663 expression is significantly upregulated in HL-60, MV4-11, and SHI-1 cells. M and U represent MSP results using primer sets for methylated and unmethylated miR-663 genes, respectively. (B) The transcript level of miR-663 is upregulated in cells treated with 5-Aza compared to DMSO: 4.5-fold in HL-60 cells (5-Aza: 7.76 vs. DMSO: 1.72; P = 0.007); 5.9-fold in MV4-11 cells (5-Aza: 2.26 vs. DMSO: 0.38; P = 0.008); and 10.6-fold in SHI-1 cells (5-Aza: 3.93 vs. DMSO: 0.37; P = 0.004).
Yan-Fang et al. BMC Medical Genetics 2013 14:74 doi:10.1186/1471-2350-14-74