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The promoter of miR-663 is hypermethylated in Chinese pediatric acute myeloid leukemia (AML)

Tao Yan-Fang1, Ni Jian2, Lu Jun1, Wang Na1, Xiao Pei-Fang1, Zhao Wen-Li1, Wu Dong1, Pang Li1, Wang Jian1, Feng Xing1* and Pan Jian12*

Author Affiliations

1 Department of Hematology and Oncology, Children’s Hospital of Soochow University, Suzhou, China

2 Translational Research Center, Second Hospital, The Second Clinical School, Nanjing Medical University, Nanjing, China

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BMC Medical Genetics 2013, 14:74  doi:10.1186/1471-2350-14-74

Published: 19 July 2013

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Additional file 1:

Analysis of promoter methylation of miRNAs in pediatric AML using NimbleGen Human DNA Methylation 385 K Promoter Plus CpG Island Arrays. We had previously analyzed the methylation status of 63 miRNAs in four pediatric AML samples (M1, M2, M3, and M4) and three NBM samples (C1, C2, and C3) using NimbleGen Human DNA Methylation Arrays. Each red box represents the number of methylation peaks (PeakScore) overlapping the promoter region for the corresponding miRNA. The PeakScore is defined as the average -log10 (P-value) from probes within the peak. The scores reflect the probability of positive methylation enrichment. The DNA methylation array analysis shows that the promoter of miR-663 is significantly methylated in AML samples (4/4), and unmethylated in NBM samples (0/3).

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Additional file 2:

Bisulfite genomic sequencing of leukemia cells. The bisulfite genomic sequencing (BGS) primers (from +86 to +384) include 21 CpGs. The amplified BGS products were TA-cloned and five to six randomly chosen colonies were sequenced. The DNA sequences of six leukemia cells are presented.

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