Relative quantification of dystrophin transcript by real-time PCR.A) Histograms represent total transcript level of dystrophin gene in 8 female carriers, calculated on dystrophin exon 12 and exon 55, in respect to two control females (their mean value referred as 100%). Error bars represent mean ± SD. B) Transcriptional level of wild-type dystrophin allele, calculated from the relative ratio of wild-type and mutated transcripts.
Brioschi et al. BMC Medical Genetics 2012 13:73 doi:10.1186/1471-2350-13-73