Table 1

Primer sequences and restriction-product characteristics
Oligo name Oligo sequence PCR size (bp) Restriction enzyme Restriction products (bp)
    FNP1 -8CG
Fw R/P 5’CCAGTTCCTTGCACTCCTG-3’ 129 BstUI (60°C) 85+44 (Pol)
Rv R/P 5’CATCCTCTCTGGCGGTTG-3’ [Bio]
Sq 5’AGAGCCAGCGGGGTC-3’
    HFE C282Y
Fw R 5’-TGGCAAGGGTAAACAGATCC-3’ 387 RsaI (37°C) 247+140 (wt)
Rv R 5’-CTCAGGCACTCCTCTCAACC-3’
Fw P 5’-CGAACCTAAAGACGTATTGCC-3’
Rv P 5’-CCCAATAGATTTTCTCAGCTCCT-3’ [Bio]
Sq 5’GGAAGAGCAGAGATATACG-3’
    HFE H63D
Fw R 5’-ACATGGTTAAGGCCTGTTGC-3’ 207 BclI (50°C) 137+70 (Pol)
Rv R 5’-GCCACATCTGGCTTGAAATT-3’
Fw P 5’-CCACATCTGGCTTGAAATTCT-3’
Rv P 5’-GTTTGAAGCTTTGGGCTACG-3’ [Bio]
Sq 5’GGGCTCCACACGGCG-3’
    TF P570S
Fw R 5’-GCTGTGCCTTGATGGTACCAGGTAA-3’ 110 BstEII (60°C) 89+21 (wt)
Rv R 5’-GGACGCAAGCTTCCTTATCT-3’
Fw P 5’-GAAAAAGACTATGAGTTGCTGTGC-3’
Rv P 5’-CTGTGACCACAGCGTGATTC-3’ [Bio]
Sq 5’-TGATGGTACCAGGAA-3’
    HEPC -582AG
Fw R 5’-ACCCTCCTGCCTTGGCCTC-3’ 252 HpyCH4IV (37°C) 226+26 (Pol)
Rv R 5’-CCATTGCTTTAAGCTCTCACC-3’
Fw P 5’-ACATCTCAAGGGTCTGACACTGG-3’
Rv P 5’-GAGCAGGGCAAGCATCAGC-3’ [Bio]
Sq 5’-TCTGACACTGGGAAAAC-3’

Fw and Rv indicate the forward and reverse primer, respectively; Sq indicates the sequencing primer; R and P indicate Restriction and Pyrosequencing technique, respectively; WT and Pol, indicate the wild-type (common) and polymorphic (rare) allele, respectively; [Bio], indicate the biotinylated primer.

Gemmati et al.

Gemmati et al. BMC Medical Genetics 2012 13:70   doi:10.1186/1471-2350-13-70

Open Data