Modified Melting Curve Analysis for DIP rs3832406. The Sensor probe design for detection of all three alleles of DIP rs3832406 is shown. The Sensor probe is designed to perfectly match the complement of Allele 3. A. Sensor probe bound to Allele 1 has a Tm of 58.8°C. B. Sensor probe bound to Allele 2 has a Tm of 60.3°C. C. Sensor probe bound to Allele 3 has a Tm of 63°C. D. Examples of homozygote melting peaks for each of the three alleles. E. Examples of heterozygote melting peaks for all three allele combinations.
Minguzzi et al. BMC Medical Genetics 2012 13:29 doi:10.1186/1471-2350-13-29