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Open Access Highly Accessed Research article

Combined analysis of three genome-wide association studies on vWF and FVIII plasma levels

Guillemette Antoni123, Tiphaine Oudot-Mellakh2, Apostolos Dimitromanolakis3, Marine Germain12, William Cohen45, Philip Wells6, Mark Lathrop7, France Gagnon3, Pierre-Emmanuel Morange45 and David-Alexandre Tregouet12*

Author Affiliations

1 UMR_S 937, INSERM, Boulevard de l'Hopital, Paris, 75013, France

2 UMR_S 937, ICAN Institute, Université Pierre et Marie Curie, Boulevard de l'Hopital, 75013, Paris, France

3 Dalla Lana School of Public Health, University of Toronto, College Street, Toronto, M5T 3M7, Ontario, Canada

4 UMR_S 626, INSERM, rue Saint-Pierre, Marseille, 13385, France

5 UMR_S 626, Université de la Méditerranée, rue Saint-Pierre, Marseille, 13385 France

6 Department of Medicine, Ottawa Hopital Research Institute, Carling Avenue, Ottawa, K1Y 4E9, Ontario, Canada

7 Institut de Génomique, Centre National de Génotypage, Commissariat à l'Energie Atomique, rue Gaston Crémieux, Evry, 91057, France

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BMC Medical Genetics 2011, 12:102  doi:10.1186/1471-2350-12-102

Published: 2 August 2011

Abstract

Background

Elevated levels of factor VIII (FVIII) and von Willebrand Factor (vWF) are well-established risk factors for cardiovascular diseases, in particular venous thrombosis. Although high, the heritability of these traits is poorly explained by the genetic factors known so far. The aim of this work was to identify novel single nucleotide polymorphisms (SNPs) that could influence the variability of these traits.

Methods

Three independent genome-wide association studies for vWF plasma levels and FVIII activity were conducted and their results were combined into a meta-analysis totalling 1,624 subjects.

Results

No single nucleotide polymorphism (SNP) reached the study-wide significance level of 1.12 × 10-7 that corresponds to the Bonferroni correction for the number of tested SNPs. Nevertheless, the recently discovered association of STXBP5, STX2, TC2N and CLEC4M genes with vWF levels and that of SCARA5 and STAB2 genes with FVIII levels were confirmed in this meta-analysis. Besides, among the fifteen novel SNPs showing promising association at p < 10-5 with either vWF or FVIII levels in the meta-analysis, one located in ACCN1 gene also showed weak association (P = 0.0056) with venous thrombosis in a sample of 1,946 cases and 1,228 controls.

Conclusions

This study has generated new knowledge on genomic regions deserving further investigations in the search for genetic factors influencing vWF and FVIII plasma levels, some potentially implicated in VT, as well as providing some supporting evidence of previously identified genes.