Table 2

PCR-RFLP analysis of the SPINK5 gene SNPs

Polymorphic Site

Primers

PCR products

Restriction enzyme

-206 G>A

5'-TCTGCCTGCTCCAACTAA-3'

5'-CACTGACACTGTGGCTATCTT-3'

674 bp

Kpn I

1103A>G (Asn368Ser)

5'-TCTGCCAATGTAGATGTT-3'

5'-CTCTAATGACTCCCAAGC-3'

670 bp

Bts I

1156G>A (Asp386Asn)

As above

670 bp

Mbo I

1258G>A (Glu420Lys)

5'-TTTTAGCCAAGCAGAAGAAG-3'

5'-CAGTTTTAAGGAATGCACGT-3

177 bp

Hinf I

2475G>T(Glu825Asp)

5'-TGGGGAACTGAAGAGCA-3'

5'-GGGACATTATTTTGCCTATC-3'

688 bp

BstF5I

Liu et al. BMC Medical Genetics 2009 10:59   doi:10.1186/1471-2350-10-59