Flowchart for section-image level processing. Original image is processed to identify the spatial positions of thousands of cell nuclei in each section image. This information is then used to compute nuclei density at each pixel of the section image using a square window (50 pixels × 50 pixels). The resulting histogram undergoes adaptive segmentation to identify regional maxima indicating high-nuclei density regions.
Petushi et al. BMC Medical Imaging 2006 6:14 doi:10.1186/1471-2342-6-14