Illustration of sampling error for small regions of interest. Horizontal axis represents true area and vertical axis represents area based on counting pixels included in the ROI. The error depends on the pixel resolution. Open circles denote a pixel resolution of 1 mm, diamonds indicate a pixel resolution up-sampled to 0.5 mm, and plus signs indicate a pixel resolution up-sampled to 0.25 mm.
Heiberg et al. BMC Medical Imaging 2010 10:1 doi:10.1186/1471-2342-10-1