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Open Access Research article

Lack of association between polymorphisms of MASP2 and susceptibility to SARS coronavirus infection

Yan Wang12, Jiangwei Yan3, Yuling Shi4, Ping Li1, Chuanxuan Liu1, Qingjun Ma1, Ruifu Yang5, Xiaoyi Wang5, Lina zhu2, Xiao Yang2 and Cheng Cao1*

Author Affiliations

1 Beijing Institute of Biotechnology, Beijing, PR China

2 Affiliated Bayi Children's Hospital, General Hospital of Beijing Command of the People's Liberation Army, Beijing, PR China

3 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, PR China

4 General Hospital of Guangzhou Command of the People's Liberation Army, Guangzhou, PR China

5 Beijing Institute of Microbiology and Epidemiology, Beijing, PR China

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BMC Infectious Diseases 2009, 9:51  doi:10.1186/1471-2334-9-51

Published: 1 May 2009

Abstract

Background

The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.

Methods

Thirty individuals with SARS were chosen for analysis of MASP2 polymorphisms by means of PCR direct sequencing. Tag single nucleotide polymorphisms (tagSNPs) were chosen using pairwise tagging algorithms. The frequencies of four tag SNPs (rs12711521, rs2261695, rs2273346 and rs7548659) were ascertained in 376 SARS patients and 523 control subjects, using the Beckman SNPstream Ultra High Throughput genotyping platform.

Results

There is no significant association between alleles or genotypes of the MASP2 tagSNP and susceptibility to SARS-CoV in both Beijing and Guangzhou populations. Diplotype (rs2273346 and rs12711521)were analyzed for association with susceptibility to SARS, no statistically significant evidence of association was observed. The Beijing and Guangzhou sample groups were homogeneous regarding demographic and genetic parameters, a joined analysis also showed no statistically significant evidence of association.

Conclusion

Our data do not suggest a role for MASP2 polymorphisms in SARS susceptibility in northern and southern China.