Table 3

List of differentially expressed cell cycle genes >2 fold in advanced HCV relative to early HCV

Gene symbol

Average Ct Adv. HCV

Average Ct Early HCV

T-test

p-value

Fold change


Cell proliferation genes


CCND1

32.65

31.00

0.14

-2.22

CCND2

31.3

32.35

0.05

5.00

CDK2

32.1

30.3

0.6

-2.46


CDK inhibitors


CDKN2B

32.4

33.50

0.04

3.03

CDKN3

34.20

32.73

0.05

5.46

CDKN1B

29.4

27.7

0.5

-2.3


G2/M checkpoint gene


KPNA2

30.85

31.3

0.98

2.75

HERC5

33.05

29.3

0.217

-8.70


DNA damage response genes


ATR

31.9

35

0.30

10.50

RAD1

30.4

32.8

0.05

6.46

HUS1

27.55

29.65

0.05

6.06

RAD17

28.45

30.35

0.057

5.27

RPA3

33.3

35.0

0.01

4.6

*TP53

30.80

27.03

0.04

-2.79


Mitotic checkpoint regulators


MAD2L1

35

32

0.04

-5.6

CDC34

32.65

30.00

0.025

-4.4


Cell cycle regulation


CCNH

32.85

35.0

0.5

5.00

CDK8

28.7

30.5

0.07

4.92

CCNG2

27.8

29.85

0.09

5.86

CKS2

30.7

32.15

0.23

3.86

CUL1

30.6

28.3

0.65

-3.48

TFDP2

28.2

30

0.08

4.92

CCNT1

28.6

31.15

0.08

4.59


The values present average Ct values without normalization and fold differences observed in Real time PCR and p-values of T-test (values ≤ 0.05 are in bold).

Sarfraz et al. BMC Infectious Diseases 2009 9:125   doi:10.1186/1471-2334-9-125

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