Table 2

List of differentially expressed cell cycle genes >2 fold in early HCV relative to normal liver.

Gene Symbol

Average Ct Early HCV

Average Ct Normal

T-test

p-value

Fold change


Cell cycle progression/cell proliferation genes


MCM2

30.85

33.4

0.05

7.670

CDC2

32.5

34.15

0.36

4.79

MCM5

28.15

29.5

0.16

3.390

MCM3

28.0

29.1

0.11

3.100

CCNE1

33.6

34.9

0.05

4.76


CDK Inhibitors


CDKN1A

27.5

28.2

0.258

2.24

CDKN1B

27.65

28.6

0.034

2.36


G2/M checkpoint gene


GADD45A

27.15

29.8

0.03

9.000

DNM2

31.75

29.6

0.005

-2.65

KPNA2

31.3

29.25

0.169

-3.730


Mitotic checkpoint genes


KNTC1

31.6

34.4

0.04

9.19

CKS1B

31.0

33.5

0.17

7.200

MAD2L1

32

34.55

0.077

7.07

CDC34

30.0

31.3

0.35

3.4

CDC16

32.6

30.8

0.027

-2.16


DNA damage response genes


CHEK1

32.35

33.70

0.24

3.09

HUS1

29.65

27.05

0.06

-4.37


Cell cycle Regulators


HERC5

29.3

33.7

0.07

20.393

CCNG2

29.85

32.3

0.11

6.400

RBBP8

31.7

30

0.108

-2.300

BCL2

29.65

31.60

0.052

3.10

UBE1

29.55

27.4

0.206

-3.500


The values present average Ct values without normalization and fold differences observed in Real time PCR and p-values of T-test (values ≤ 0.05 are in bold).

Sarfraz et al. BMC Infectious Diseases 2009 9:125   doi:10.1186/1471-2334-9-125

Open Data