Table 2

Oligonucleotides (5'-3') used in this study

Oligonucleotide

Sequence

Orientationa

Application


tir:1U19

atg cct att ggt aat ctt g

Sense

tir 5' amplification sequencing

tir:72U17

aca aac cga cgg tgc ag

Sense

tir sequencing

tir:473L17

atg ccc agc acc acc ac

Antisense

tir sequencing

tir:521U17

aag ccc gcc aaa gga ta

Sense

tir RR1 sequencing

tir:967L17

tct tcg cct gct gct tt

Antisense

tir RR1 sequencing

tir:1046U17

cta aac gcc agg agg ag

Sense

tir RR2, 3, & 4 sequencing

tir:1572L17

ggc gta atc cac cac tt

Antisense

tir RR2, 3, & 4 sequencing

tir:1638L16

cgc tgg tgg gtt att c

Antisense

tir 3' amplification sequencing

eae:16U16

tgt tat acc cgg acc c

Sense

eae 5' amplification sequencing

eae:135U19

taa att ggg ttc gga ttc a

Sense

eae sequencing

eae:382L17

aca aga ggt gcc gaa cc

Antisense

eae sequencing

eae:490U16

aat tat gcg gca caa c

Sense

eae sequencing

eae:574L18

gtt acc agc gat acc aag

Antisense

eae sequencing

eae:626U18

att atg gaa cgg cag agg

Sense

eae sequencing

eae:886L19

ttt tga aat agt ctc gcc a

Antisense

eae sequencing

eae:1090U17

gat aag ctg cag tcg aa

Sense

eae sequencing

eae:1178L18

ttt cat tac ccg tac cat

Antisense

eae sequencing

eae:1290U18

atc agg cag ccg tta cga

Sense

eae sequencing

eae:1492L19

ttc cgc tat gct gaa tct g

Antisense

eae sequencing

eae:1695U18

gga taa gac ttc ggc taa

Sense

eae sequencing

eae:1732L18

cgt cgc ggt ata agt aat

Antisense

eae sequencing

eae:1883U17

cgc cag gac agg tcg tc

Sense

eae sequencing

eae:2135L17

att tcc cgt ggt tgc tt

Antisense

eae sequencing

eae:2249U19

ttg gta aca atg tca gag g

Sense

eae sequencing

eae:2358L13

acc acc gct tgc t

Antisense

eae sequencing

eae:2360U17

caa gcg gtg gtg atg gt

Sense

eae sequencing

eae:2699L17

tcc aga acg ctg ctc ac

Antisense

eae sequencing

eae:2787L19

tta ttc tac aca aac cgc a

Antisense

eae 3' amplification sequencing


a With respect to tir and eae mRNA.

Bono et al. BMC Infectious Diseases 2007 7:98   doi:10.1186/1471-2334-7-98

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