Open Access Correction

Correction: The burden of diarrhoea, shigellosis, and cholera in North Jakarta, Indonesia: findings from 24 months surveillance

Magdarina D Agtini1*, Rooswanti Soeharno1, Murad Lesmana2, Narain H Punjabi2, Cyrus Simanjuntak2, Ferry Wangsasaputra12, Dazwir Nurdin2, Sri Pandam Pulungsih3, Ainur Rofiq1, Hari Santoso4, H Pujarwoto1, Agus Sjahrurachman5, Pratiwi Sudarmono5, Lorenz von Seidlein6, Jacqueline L Deen6, Mohammad Ali6, Hyejon Lee6, Deok Ryun Kim6, Oakpil Han6, Jin Kyung Park6, Agus Suwandono1, Ingerani1, Buhari A Oyofo2, James R Campbell2, H James Beecham2, Andrew L Corwin2 and John D Clemens67

Author Affiliations

1 National Institute of Health Research and Development, Jakarta Indonesia, Ministry of Health, Jakarta, Indonesia

2 United States Navy Medical Research Unit 2, Jakarta, Indonesia

3 Infectious Disease Hospital Sulianti Saroso, Ministry of Health, Jakarta, Indonesia

4 Communicable Disease Control and Environmental Health, Ministry of Health, Jakarta, Indonesia

5 Department of Microbiology, University of Indonesia, Jakarta, Indonesia

6 International Vaccine Institute, Seoul, Korea

7 National Institute of Child Health and Human Development, Bethesda, Maryland, USA

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BMC Infectious Diseases 2007, 7:1  doi:10.1186/1471-2334-7-1

The electronic version of this article is the complete one and can be found online at:

Received:10 October 2006
Accepted:26 January 2007
Published:26 January 2007

© 2007 Agtini et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This is a correction of an earlier published article.


After the publication of this work[1], we became aware that the our description of the methods used to identify Shigella spp. did not describe the identification of a subset of Shigella spp. which were untypeable with commercial sera. Additional typing was conducted by Dr. Kaisar A. Talukder and coworkers (Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.

Brief description of the methods used for serotyping the untypeable S. flexneri strains using MASF

All Shigella strains which agglutinated with commercial polyvalent anti-S. flexneri serum (Denka Seiken, Tokyo, Japan) but were untypeable using commercial serotype specific antisera were examined using a panel of monoclonal antibodies against S. flexneri (MASF B) according to the typing scheme shown in Table 1 using monoclonal antibodies from one supplier (Reagensia AB, Stockholm, Sweden) with the exception of MASF 1c which was provided by Dr. N. I. A. Carlin (SBL, Sweden) [2-5]. Untypeable S. flexneri strains which agglutinated strongly with polyclonal MASF B and type antigen factor IV-2 but not with group factor Y-5 or 6 thereby excluding their characterization into serotype 4a or 4b were designated as serotype 4 if they agglutinated with a new antigenic determinant E1037 (IV-1). Untypeable strains which agglutinated with antibodies against the provisional antigen, MASF IV-1 specific for the new antigenic determinant E1037, were designated as 4X. Untypeable strains which agglutinated only with MASF Ic were designated as 1c.

Table 1. S. flexneri serotyping criteria using MASF antibodies


  1. Agtini MD, Soeharno R, Lesmana M, Punjabi NH, Simanjuntak C, Wangsasaputra F, Nurdin D, Pulungsih SP, Rofiq A, Santoso H, Pujarwoto H, Sjahrurachman A, Sudarmono P, von Seidlein L, Deen JL, Ali M, Lee H, Kim DR, Han O, Park JK, Suwandono A, Ingerani, Oyofo BA, Campbell JR, Beecham HJ, Corwin AL, Clemens JD: The burden of diarrhoea, shigellosis, and cholera in North Jakarta, Indonesia: findings from 24 months surveillance.

    BMC Infect Dis 2005, 5:89. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  2. Carlin NI, Rahman M, Sack DA, Zaman A, Kay B, Lindberg AA: Use of monoclonal antibodies to type Shigella flexneri in Bangladesh.

    J Clin Microbiol 1989, 27(6):1163-1166. PubMed Abstract | PubMed Central Full Text OpenURL

  3. Talukder KA, Dutta DK, Safa A, Ansaruzzaman M, Hassan F, Alam K, Islam KM, Carlin NI, Nair GB, Sack DA: Altering trends in the dominance of Shigella flexneri serotypes and emergence of serologically atypical S. flexneri strains in Dhaka, Bangladesh.

    J Clin Microbiol 2001, 39(10):3757-3759. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  4. Talukder KA, Islam MA, Dutta DK, Hassan F, Safa A, Nair GB, Sack DA: Phenotypic and genotypic characterization of serologically atypical strains of Shigella flexneri type 4 isolated in Dhaka, Bangladesh.

    J Clin Microbiol 2002, 40(7):2490-2497. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  5. Talukder KA, Islam Z, Islam MA, Dutta DK, Safa A, Ansaruzzaman M, Faruque AS, Shahed SN, Nair GB, Sack DA: Phenotypic and genotypic characterization of provisional serotype Shigella flexneri 1c and clonal relationships with 1a and 1b strains isolated in Bangladesh.

    J Clin Microbiol 2003, 41(1):110-117. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

Pre-publication history

The pre-publication history for this paper can be accessed here: