Table 2 |
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|
Percentage of IκB remaining in A549 after addition of high- and low-binding S. pneumoniaea |
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|
Time after addition of bacteria |
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|
|
||||
|
Treatment of A549 cellsb |
0 hours |
4 hours |
6 hours |
8 hours |
|
|
||||
|
low binders |
100% |
81.3% |
65.6% |
62.1% |
|
high binders |
100% |
60.8% |
34.9% |
25.1% |
|
|
||||
|
ANOVA p-valuec |
0.423 |
2.3 × 10-7 |
1.3 × 10-7 |
1.1 × 10-8 |
|
|
||||
|
a Combined average of IκB levels from cells treated with 7 low-binders or 7 high-binders. Each S. pneumoniae isolate was tested in triplicate thus each measurement is the average from 21 different experiments at each time point. b A549 cells were incubated with an MOI of 50 bacteria/cell for the listed time periods. Proteins from the cell culture were then harvested for the analysis of IκB degradation. c Two-way analysis of variance comparing IκB degradation in A549 treated with low binders and high binders for each time period. IκB degradation measured as described in materials and methods. |
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|
Robson et al. BMC Infectious Diseases 2006 6:71 doi:10.1186/1471-2334-6-71 |
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