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Open Access Research article

Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties

Rachel L Robson, Natalie A Reed and Rebecca T Horvat*

Author Affiliations

Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City KS 66160, USA

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BMC Infectious Diseases 2006, 6:71  doi:10.1186/1471-2334-6-71

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2334/6/71


Received:10 February 2006
Accepted:11 April 2006
Published:11 April 2006

© 2006 Robson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Adherence of Streptococcus pneumoniae bacteria to lung cells is a first step in the progression from asymptomatic carriage to pneumonia. Adherence abilities vary widely among S. pneumoniae patient isolates. In this study, the binding properties of S. pneumoniae isolates and the effects of binding on activation of the Nuclear Factor-Kappa-B (NFκB) pathway and cytokine secretion by type II pneumocytes were measured.

Methods

Mechanisms of high- and low-binding S. pneumoniae adherence to A549 cells were investigated by blocking putative receptors on bacteria and host cells with antibody and by eluting choline-binding proteins off of bacterial surfaces. NFκB activation was measured by western blot and immunocytochemistry and cytokine secretion was detected by a protein array.

Results

This study shows that S. pneumoniae isolates from pneumonia patients (n = 298) can vary by as much as 1000-fold in their ability to bind to human lung epithelial cells. This difference resulted in differential activation of the NFκB pathway. High-, but not low-binding S. pneumoniae used

    C
holine-
    b
inding
    p
rotein A (CbpA) to bind to complement component C3 on epithelial cell surfaces. Interleukin-8 (IL-8) was the only cytokine secreted by cells treated with either low- or high-binding S. pneumoniae.

Conclusion

These results indicate that S. pneumoniae clinical isolates are not homogeneous in their interaction with host epithelial cells. The differential activation of host cells by high- and low-binding S. pneumoniae strains could have implications for the treatment of pneumococcal pneumonia and for vaccine development.

Background

Streptococcus pneumoniae is a leading cause of community-acquired pneumonia [1,2]. Yet S. pneumoniae can live harmlessly in the upper respiratory tract of human hosts, and be cultured from the nasopharynx from as many as 40% of the healthy adult population [3,4].

The pathogenicity of S. pneumoniae in pneumonia has been the subject of much research [5-9]. Pneumonia caused by S. pneumoniae begins when the bacteria move from the upper respiratory tract to the lungs [10]. Although S. pneumoniae cannot adhere to healthy ciliated upper respiratory epithelium [11], the bacteria do bind to type II pneumocytes [12,13]. Replication and spread of bacteria through the lung during the first hours of acute S. pneumoniae pneumonia is followed by red hepatization, as the lung becomes engorged with blood. Cytokines recruit neutrophils to the site of infection [14]. It is during this stage that the S. pneumoniae pneumonia patient is most at risk for severe consequences [15].

The adherence of S. pneumoniae to lung epithelial cells is a critical first step in the progression from the asymptomatic carrier state to pneumonia [16]. While studies have indicated that S. pneumoniae can use host cell platelet-activating factor receptor (PAFr) as an anchor for adherence [17-19], PAFr is not the sole binding site on human epithelium used by S. pneumoniae. S. pneumoniae expresses a 75-kDa surface protein,

    C
holine-
    b
inding
    p
rotein
    A
(CbpA) which has been shown to bind to host cells via secreted complement component C3 [20-22]. The binding of C3 on host cells can activate those cells and trigger cytokine release, notably release of the neutrophil-recruiting interleukin-8 (IL-8) [20,23]. Type II pneumocytes have long been known to synthesize and secrete complement component C3 [24], providing a target for S. pneumoniae adherence to those cells via CbpA.

It has been noted that 75% of S. pneumoniae strains possess genes for CbpA [25] and thus have the potential to bind to C3. This study shows that clinical isolates vary widely in their ability to adhere to lung epithelial cells. A survey of 298 patient isolates from the University of Kansas Medical Center between 1996 and 2001 showed that S. pneumoniae strains vary as much as 1000-fold in their ability to bind to a human type II pneumocyte cell line (Robson et al. poster B316, presented at 105th American Society for Microbiology conference, Atlanta, GA, June 2005). Further, high-binding S. pneumoniae were selected from a low-binding parent strain, producing two genetically-similar strains that vary in adherence by 2–3 log10. The S. pneumoniae high binders selected from low-binder parent strains have the same pulsed-field gel electrophoresis patterns and serotypes as their low-binding precursors (Robson et al. poster B75, presented at 102nd American Society for Microbiology conference, Salt Lake City, UT, May 2002)

In this study, the binding properties of naturally-occurring and artificially-evolved high- and low-binding S. pneumoniae isolates are compared and the effects of binding on activation of the

    N
uclear
    F
actor-Kappa-B (NFκB) pathway and Interleukin-8 (IL-8) secretion by type II pneumocytes are measured. Other studies have examined the impact of pneumococcal adherence on the activation of lung epithelial cells; however this is the first to evaluate a substantial number of patient isolates for binding activity, and the first to examine differences between high- and low-binding S. pneumoniae in the activation of lung epithelial cells.

Methods

Cell culture

A549, a human pneumocyte II cell line, was obtained from ViroMed. Commercially prepared shell vials, containing A549 on glass coverslips, were used for immunocytochemistry. Cells used in other applications were transferred and grown to confluence on sterile culture dishes (Midsci) in commercially-prepared Dulbecco's Modified Eagle's Medium (DMEM) (Sigma) with 10% fetal bovine serum (Atlanta Biologicals) and 1% L-glutamine/penicillin-streptomycin, 1% amphotericin B and 1% MEM essential vitamin mix (Sigma). Cells were transferred to 24-well plates for adherence assays or polystyrene culture dishes for Western blot samples. Cells from passages 4 through 7 were used in all applications.

Identity of S. pneumoniae strains

S. pneumoniae isolates were collected from patients at the University of Kansas Medical Center from 1996–2001. All S. pneumoniae isolates in this study were from blood or respiratory specimens of hospitalized patients with pneumonia. Medical records were retrospectively reviewed for signs and symptoms of pneumonia. Pneumonia was defined as the presence of a new pulmonary infiltrate by chest radiograph, confirmed by a radiologist as compatible with pneumonia.

Additionally the patient must have had at least two signs or symptoms consistent with a lower respiratory tract infection: temperature of 38°C or greater, new onset or increased cough, purulent sputum, crackles, or dyspnea. Isolates from patients with other diagnoses were not included in this study. The Human Subjects Committee at the University of Kansas Medical Center approved the study protocol and the information was recorded in a manner that preserved patient confidentiality. These procedures are in accordance with the guidelines for human experimentation of the U.S. Department of Health and Human Services.

Naturally-occurring high- and low-binders were chosen from these patient isolates for further experiments. Evolved high-binding S. pneumoniae were descendants of low-binding strains: two patient isolates, serotypes 3 and 18C, and ATCC 6301, serotype 1. The reference strain ATCC 6301 was obtained from the American Type Culture Collection.

All S. pneumoniae used except for ATCC 6301 had different genetic backgrounds, as determined by pulsed-field gel electrophoresis using established techniques [26]. Artificially-evolved high-binding strains had the same PFGE pattern and serotype as their respective low-binding parent strains.

Adherence assay and selection for adherence

24-well plates containing 200,000 A549 cells per well were washed three times with Muller-Hinton broth (MHB) (Remel) at room temperature. After washing, each well was inoculated with 107 bacteria in 1 mL additive-free DMEM, and incubated at 37°C, 5% CO2 for two hours. The cells were then washed three times with room temperature MHB, and cells were lysed with 0.02% trypsin (Sigma) for 30 minutes. Bacterial viability was not affected by 0.02% trypsin. The A549 cell lysate was then diluted and plated on sheep blood agar (SBA) (Remel), using a standard spread plate technique. Colony counts from the cell lysate dilutions determined the number of adherent bacteria per 100 A549 cells.

The evolved high binding strains of S. pneumoniae were derived from low binding parent strains by using sequential passage on fresh A549 monolayers. When selecting for adherence, the adherent bacteria from the previous passage on A549 cells were used to inoculate new cell layers of A549 for subsequent passages.

In choline-elution experiments, bacteria were soaked in sterile 2% choline (Sigma) in saline for 20 minutes, centrifuged and resuspended in sterile saline before adding the bacteria to A549 cells in 24-well plates.

In all adherence experiments, A549 viability immediately prior to the final treatment with 0.02% trypsin was over 90%, as determined by trypan blue exclusion.

Blocking of receptors on S. pneumoniae and A549

The CbpA proteins on S. pneumoniae were blocked by soaking bacteria in sterile saline supplemented with either1:100 rabbit anti-CbpA, or 1:100 normal rabbit serum as the negative control, for 30 minutes at room temperature with gentle shaking. The bacteria were then washed in sterile saline prior to being added to A549 cells to assess adherence.

To block the C3 and PAF-receptor on the A549 cells, the epithelial cells were treated with 1:100 rabbit anti-human C3 antibody, 1:100 goat anti-human PAFr antibody (Santa Cruz Biotechnology), or 1:100 normal rabbit serum as the negative control for 2 hours. The cells were then washed with MHB and inoculated with a standard suspension of bacteria.

Serotyping

Serotyping was performed using bead agglutination, as previously described [27].

Western blot for IκB

Protein preparations from A549 cells treated with high- and low-binding bacterial strains (MOI = 50 bacteria/cell) were quantified using a protein quantification kit (BioRad). 50 μg of protein was electrophoresed in a 30% polyacrylamide gel, transferred to a polyvinylidine fluoride (PVDF) membrane (BioRad), Ponceau stained, blocked in 5% nonfat dry milk (NFDM) in Tris-buffered saline-Tween (TBST), and probed with rabbit anti-human IκB (Santa Cruz Biotechnology) in 5% NFDM-TBST, with horseradish-peroxidase (HRP) conjugated donkey anti-rabbit IgG (Santa Cruz Biotechnology) used as a secondary antibody. IκB bands were visualized using ECL substrate (Amersham Biosciences) and autoradiography.

Computerized densitometry was used to analyze changes in IκB band density relative to negative controls. Membranes were stripped and reprobed with mouse anti-human actin antibody to confirm that equivalent amounts of total protein had been loaded into each well.

Western blot for eluted CbpA

Choline-binding proteins were eluted from S. pneumoniae as described above. Supernatants were collected and sterile-filtered and proteins were precipitated using a standard tricholoracetic acid technique. The proteins were electrophoresed on a 30% polyacrylamide gel and transferred to PVDF as described above. Membranes were Ponceau stained, blocked in 5% NFDM-TBST, and probed with 1:100 rabbit anti-CbpA (kindly provided by Drs. Elaine Tuomanen and Margaret Hostetter). HRP-conjugated donkey anti-rabbit IgG was used as a secondary antibody.

Immunocytochemistry

A549 cells grown on glass coverslips in shell vials (ViroMed) were used for immunocytochemistry. Cell layers were washed with MHB and inoculated with 107 bacteria per vial, as in adherence assays. At the end of the incubation period (4, 6, or 8 hours at 37°C, 5% CO2), cell layers were washed three times with ice-cold phosphate-buffered saline (PBS) and fixed overnight at 4°C in 5% formalin in PBS. Immunocytochemistry was conducted using a kit (BioGenex) with 1:25 rabbit anti-NFκB (Santa Cruz Biotechnology) used as a primary antibody. Slides were examined at 400 x.

Cytokine array

Supernatants from A549 cells treated with high and low binding S. pneumoniae isolates were assayed for the presence of 42 human cytokines using a RayBio® Human Cytokine Antibody Array III, following manufacturer's instructions (Raybiotech). Cytokine array luminesence was visualized with ECL substrate and autoradiography.

Measurement of IL-8 concentrations

IL-8 was measured from the supernatant of treated epithelial cells using the Bio-plex suspension array system with fluorescently dyed Luminex microspheres (BioRad). Culture supernatant was collected from cells at 30 minutes, 1 hour, 2 hours, 4 hours, 6 hours and 8 hours after inoculation with bacteria. The culture supernatant was stored at -70°C until tested. Assays were performed in a 96-well filtration plate (Millipore) with 5,000 beads coated with human IL-8 antibody. The standards, samples or blanks were mixed with beads in a final volume of 100 μl and incubated for 30 minutes at 25°C with continuous shaking. After three washes, biotinylated antibodies were added for 30 minutes. Beads were again washed three times and streptavidin-phycoerythrin was added for 10 minutes. The fluorescence intensity of the beads was measured using the Bio-Plex array reader. Bio-Plex Manager software was used for data analysis. The level of IL-8 detection was 7.8 pg/ml. IL-8 standards were assayed in triplicate with each assay; n = 7 samples run per treatment per timepoint.

Statistical analysis

All statistical analysis was done in Statistical Analysis Software (SAS). Graphs were made using Microsoft Excel.

Pulsed-field gel electrophoresis

Genomic DNA from artificially-evolved and naturally occurring S. pneumoniae strains was prepared as previously described [26]. The fragments were resolved using a GenePath System (BioRad) set on the "Enterococcus" running program.

Results

S. pneumoniae clinical isolates vary in ability to bind to epithelial cells

Clinical isolates of S. pneumoniae (n = 298) were assayed for ability to bind to A549 cells, a human type II pneumocyte cell line. Bacterial isolates varied by more than 1000-fold in their ability to adhere to cells. Table 1 summarizes S. pneumoniae clinical isolates' adherence abilities into 4 distinct categories: low, low-moderate, moderate and high. The majority (66%) of S. pneumoniae assayed were low binders, adhering to lung cells at an average rate of 0.82 ± 0.52 bacteria per 100 A549 cells. A small minority (2%) of S. pneumoniae were high binders, adhering to lung cells with an average rate of 2,360 ± 770 bacteria bound per 100 cells. The remainder of the isolates were low-moderate or moderate binders. Differences in adherence ability among the four categories was statistically significant (one-way ANOVA p-value << 0.005; F = 167.84 where Fcritical = 2.74) and reproducible. Neither serotype nor binding ability predicted for invasive disease, defined here as the isolate having been cultured from blood (Cochran-Mantel-Haenszel test, p = 0.41 and p = 0.82, respectively). No relationships were observed between serotype and binding ability, or between antibiotic resistance and binding ability of S. pneumoniae.

Table 1. Observed adherence properties of naturally-occurring S. pneumoniae clinical isolates

High-binding S. pneumoniae isolates use CbpA to bind to C3 on epithelial cell surfaces

To investigate pneumocyte receptors used by high- and low-binding S. pneumoniae, PAFr and C3 on the A549 cells were blocked with specific antibodies. Pre-treating A549 cells with anti-PAFr antibody did not reduce adherence of high-binding S. pneumoniae strains (data not shown). However, adherence of low-binding S. pneumoniae strains to A549 cells was slightly reduced (40%) by blocking PAFr on host cells with antibody (data not shown).

Blocking cell surface-bound complement component C3 with anti-human C3 antibody significantly reduced adherence of all but one high binding S. pneumoniae strain (Figure 1A). Alternatively, only one low-binding S. pneumoniae strain bound less avidly to A549 cells treated with anti-C3 antibody than to control cells (Figure 1B).

thumbnailFigure 1. Adherence of high-binders, but not low-binders, is reduced by blocking CbpA or C3. Solid bars, negative control; open bars, bacteria pre-treated with anti-CbpA antibody; dotted bars, A549 cells pre-treated with anti-C3 antibody. Negative control A549 cells pre-treated with normal rabbit serum. All values are mean ± one standard deviation, based on three independent experiments. Mean values are above each bar. The Y-axis units differ on graphs 1A and 1B. 1A. High-binding S. pneumoniae are identified by serotype along X-axis. 6301 evolved, 3 evolved, and 18C evolved are high-binding descendants of low-binding parent strains. Isolates identifed as 6 B all share that serotype but have different PFGE patterns. 1B. Low-binding S. pneumoniae strains are identified by serotype along X-axis. 6301 parent, 3 parent, and 18C parent are initial low-binding strains of 6301 evolved, 3 evolved, and 18C evolved.

Since choline-binding proteins (Cbps) on bacteria have been implicated in S. pneumoniae adherence to host cells, their involvement in the differential adherence of S. pneumoniae strains was investigated. All Cbps were eluted off of the surfaces of high- and low-binding S. pneumoniae using standard methodology [28]. Elution of choline-binding proteins from S. pneumoniae surfaces eliminated bacterial adherence to A549 cells, among both high-binding and low-binding strains. Thus, choline-binding proteins are necessary for adherence of both high- and low-binding S. pneumoniae to epithelial cell surfaces.

To determine whether CbpA is responsible for S. pneumoniae adherence to A549 cells, bacteria were treated with anti-CbpA (provided by Drs. Hostetter and Tuomanen). Similar to blocking host cell C3 with anti-C3 antibody, blocking pneumococcal CbpA with anti-CbpA antibody significantly reduced adherence of all but one high-binding S. pneumoniae strain to A549 cells (Figure 1A). Blocking CbpA on low-binding S. pneumoniae with antibody did not significantly reduce adherence to A549 cells (Figure 1B).

The removal of CbpA from bacterial surfaces by elution in 2% choline solution was confirmed by Western blot. Membranes were probed with anti-CbpA. Eluents from all high-binding and all but one low-binding S. pneumoniae strains exhibited CbpA-positive bands at 75 kDa. These data indicate that all but one isolate used in these experiments express CbpA, and that this protein was successfully removed from bacterial surfaces by choline elution (data not shown).

High-binding S. pneumoniae activate NFκB pathway

Activation of the NFκB pathway in A549 was detected by the degradation of the NFκB inhibitor, IκB. Figure 2 shows Western blots for IκB from A549 cells treated with high- and low-binding S. pneumoniae strains at different time points. Both high- and low-binding S. pneumoniae induced IκB degradation in A549 cells. However, at the 4, 6 and 8 hour time points, IκB levels were significantly lower among all cells treated with high-binding S. pneumoniae compared to all cells treated with low-binding strains (two-way ANOVA comparison of IκB band densities for cells treated with high and low binders at 4, 6, and 8 hours relative to zero timepoint). Table 2 summarizes statistical analysis of IκB degradation in A549 after exposure to high-and low-binding S. pneumoniae strains. Figure 2A shows IκB loss induced by an artifically-evolved high-binder and its genetically-similar low-binding parent strain. Figure 2B shows IκB loss induced by two unrelated high- and low-binder clinical strains. Binding of C3 on A549 surfaces by anti-C3 antibody did not induce IκB degradation.

Table 2. Percentage of IκB remaining in A549 after addition of high- and low-binding S. pneumoniaea

thumbnailFigure 2. High-binders induce prolonged degradation of IκB compared to low-binders. All values are mean ± one standard deviation, based on three independent experiments. 2A. IκB degradation induced by serotype 3 evolved high-binder and 3 low-binding parent strains. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB are shown below each line graph. 2B. IκB degradation induced by serotype 9N low-binding clinical isolate and serotype 6B high-binding clinical isolate. Closed squares, IκB level relative to time zero in A549 treated with low-binder. Open triangles, IκB level relative to time zero in A549 treated with high-binder. Representative Western blots for IκB shown below line graph.

Immunocytochemistry confirmed the activation of NFκB pathway by demonstrating the migration of NFκB from the cytoplasm into the nucleus in A549 cells treated with high-binding, but not low binding, strains (Figure 3).

thumbnailFigure 3. NFκB migrates to the nucleus after cell interaction with high-binders, but not low-binders, 4 hours after inoculation with bacteria. NFκB is detected as a dark brown stain against a pale lavender background. 3A, negative control. 3B, A549 incubated with serotype 3 low-binders (Black arrow shows NFκB protein in the cytoplasm). 3C, A549 incubated with serotype 3 high-binders (White arrow shows NFκB protein detected in the nucleus).

Cytokine activation by high and low binding S. pneumoniae

Cytokine release was evaluated using a protein array that included 42 cytokines. Culture fluid from A549 cells inoculated with high- and low-binding S. pneumoniae was tested after 2 hours of incubation. The only cytokine detected after exposure to either high- or low-binding strains was IL-8 (Figure 4). To determine whether high- or low-binding S. pneumoniae induced different amounts of IL-8 secretion from A549 cells, a Luminex bead system was used. No significant difference in IL-8 concentrations was observed in the supernatants of cells treated with high- versus low-binders at any time point prior to 8 hours. Cells treated with low-binders released significantly more IL-8 at 8 hours than did those treated with high-binders (Wilcoxon Rank-Sum, two-tailed p-value < 0.05). Negative controls induced only negligible IL-8 secretion, near the detection limit of the system (1.6 pg/mL). Measurement of other cytokines, TNF-α and IL-4, was below the detection limit of the system at all time points for all cells.

thumbnailFigure 4. Supernatants from A549 cells treated with high- and low-binding S. pneumoniae were evaluated with a RayBio® Human Cytokine Antibody Array III, which detects 42 different human cytokines. 1A and 2A, positive control; 3A and 4A, negative control; 5A, ENA-78; 6A, GCSF; 7A, GM-CSF, 8A, GRO; 9A, Gro-α; 10A, I-309; 11A, IL-1α; 12A, IL-1β; 1B, IL-2; 2B, IL-3; 3B, IL-4; 4B, IL-5; 5B, IL-6; 6B, IL-7; 7B, IL-8; 8B, IL-10; 9B, IL-12; 10B, IL-13; 11B, IL-15; 12B, IFN-γ; 1C, MCP-1; 2C, MCP-2; 3C, MCP-3; 4C, MCSF; 5C, MDC; 6C, MIG; 7C, MIP-1; 8C, RANTES; 9C, SCF; 10C, SDF-1; 11C, TARC; 12C, TGF-β; 1D, TNF-α; 2D, TNF-β; 3D, EGF; 4D, IGF-1; 5D, angiogenin; 6D, oncostatin M; 7D, thrombopoietin; 8D, VEGF; 9D, PDGF; 10D, leptin; 11D, negative control; 12D, positive control. The positive result for IL-8, in position 7B, with negative results for all other cytokines, was observed for A549 cells treated with both high- and low-binding bacteria. Experiment replicated with four different high-binding, and four different low-binding, S. pneumoniae strains.

Discussion

Adherence to host cells is the first step in the progression from S. pneumoniae carriage to disease [3]. While adherence to host cells by S. pneumoniae has been shown in a number of studies [10,12,17,20-22,29,30], this is the first investigation to compare adherence across a large sample of S. pneumoniae isolates from pneumonia patients. An important conclusion of this study is that S. pneumoniae isolates' ability to adhere to lung cells vary by more than 1,000-fold. Additionally, S. pneumoniae with higher binding activity to host cells have a prolonged activation of the NFκB pathway.

Patients infected with high-binding isolates could be at increased risk compared to patients infected with the more common low-binding isolates. While high-binding S. pneumoniae strains are relatively uncommon among patient isolates, they could still be important clinically. The United States' Centers for Disease Control expects at least 100,000 cases of pneumococcal pneumonia every year in the United States alone [31]. Thus, 2,000 of those patients would be expected to be infected with high-binding strains if the percentages reported here are representative of S. pneumoniae isolates overall. Although this investigation does not compare outcomes of patients infected with high- and low-binding S. pneumoniae, studies are in progress to evaluate this association. These studies require the evaluation of more patients since high-binding bacteria comprise such a small minority (2%) of patient isolates.

There was no correlation between isolate ability to bind to host cells and serotype, although other studies have shown a relationship between pathogenicity and serotype [32] and invasiveness and serotype [33]. In this study, 18 serotypes were found among 298 patient isolates. While 3 of 6 high-binding isolates were serotype 6B, this capsular type was not statistically linked to increased adherence, since 6B is a common serotype in this sample (n = 39; 13%).

High-binding S. pneumoniae used a bacterial surface protein, CbpA, to bind to C3 on lung epithelial cells, while low-binding strains did not use CbpA or C3. This interaction between CbpA and C3 has been noted by others [21,22]. CbpA is also capable of binding to other cellular receptors, such as the polymericimmuno globulin receptor (pIgR) on nasopharyngeal epithelial cells [30,34] and PAFr [17,29]. In this study blocking host PAFr did not reduce the adherence of high-binders to A549 cells.

Orihuela et al. observed that CbpA is required for nasopharyngeal colonization, progression from the nasopharynx to the lower respiratory tract, and the progression from bacteremia to meningitis in a mouse model but is not needed for S. pneumoniae survival in murine lungs or blood [35]. In contrast, Paton and Berry showed that CbpA does not contribute to S. pneumoniae pathogenicity in mice infected via intraperitoneal injection [36]. Future studies are needed to elucidate the importance of CbpA on virulence in humans infected with S. pneumoniae.

Others have shown that high-binding S. pneumoniae strains have a transparent colony morphology, while colonies of low-binding S. pneumoniae are likely to be opaque [12,37] Further, it has been observed that transparent S. pneumoniae strains contain more CbpA than do opaque strains [21] and that individual S. pneumoniae isolates can fluctuate between highly-adherent transparent and less-adherent opaque phases [37,38]. Phase variation was not noted when comparing high-binding S. pneumoniae isolates with low-binding S. pneumoniae isolates. All clinical strains used exhibited stable binding activity and colony morphology over at least 4 years and after numerous passages from the freezer to agar. Additionally, artificially-evolved high binders had the same colony morphology as did their low-binding progenitors. Thus, in this instance it does not seem that colony morphology was responsible for differences in binding ability of these clinical isolates. It is possible that certain colony morphotypes were selected out in the host or during the infectious process. Those studies would require additional patient isolates and extensive review of patient history. Interestingly, four of the high-binding S. pneumoniae isolates came from patients under the age of 5 years. This observation could imply that high-binding S. pneumoniae using CbpA are more prevalent in children than in adults. This interesting finding requires further study.

CbpA has been considered as a vaccine candidate. CbpA is immunogenic in rabbits and S. pneumoniae human volunteers [39,40]. Recently, Zhang et al. found that children with higher serum levels of anti-CbpA antibodies had a reduced risk of S. pneumoniae carriage [41]. Thus, CbpA-mediated binding to host cells could be an important contributor to S. pneumoniae pathogenicity and could have implications for the development of vaccines directed against CbpA.

In this study, high-binding S. pneumoniae were observed to strongly activate the NFκB pathway in lung epithelial cells. NFκB activation was independent of serotype and genetic background. Although NFκB activation often leads to secretion of many different cytokines, in this study only IL-8 was released by cells treated with either high- or low-binding S. pneumoniae. Epithelial cells surprisingly produced more IL-8 after 8 hours of incubation with low binding S. pneumoniae than with high binding isolates. This indicates differences in intracellular pathways within the epithelial cells after activation of different receptors by S. pneumoniae. Additionally, the interaction and recruitment of other cell types to the site of infection may be effected by this differential activation. A study by Jones et al. showed that IL-1 and TNF-α were rapidly released by murine lungs in response to S. pneumoniae infection [42]. Thus, although the results from the current study indicate a difference in the initial interaction of S. pneumoniae with host epithelial cells alone, further investigation in the presence of other cell types is needed to understand the implications of this difference in epithelial cell activation.

NFκB activation and IL-8 secretion by pneumocytes recruit neutrophils to the lung [43,44]. While neutrophils kill bacteria, they also damage lung tissue [44]. It is not clear whether the increased IL-8 secretion in pneumocytes treated with low-binding S. pneumoniae is ultimately protective or destructive. It is also unclear why the increased NFκB activation seen in cells treated with high-binding S. pneumoniae did not result in increased IL-8 secretion.

Conclusion

This study has shown that S. pneumoniae isolates have differences in ability to bind to human lung epithelial cells. This difference was reproducible and resulted in differential activation of the NFκB pathway and IL-8 secretion. High binding S. pneumoniae adhered via an interaction between CbpA and C3. These results indicate that S. pneumoniae are not homogeneous in their interaction with host epithelial cells and may have differences in pathogenicity. Further studies are needed to elucidate the pathogenic implication of this difference in binding and activation of epithelial cells.

Competing interests

The author(s) declare that they have no competing interests.

Authors' contributions

RR and RH conceived of the study. RR conducted all evolution of adherence experiments, pulsed-field gel electrophoresis, and all immunoblotting and immunocytochemistry. RR and NR conducted all serotyping and adherence assays. NR designed and compiled the patient database and performed statistical analyses. RH collected cytokine data.

Acknowledgements

The authors would like to thank Dr. Elaine Tuomanen and Dr. Margaret Hostetter for providing anti-CbpA antibody for use in these experiments. We would also like to thank Dr. Rukiyah Van Dross for her assistance in troubleshooting Western blots. We would like to acknowledge The Madison and Lila Self Graduate Fellowship at the University of Kansas for its financial support of Rachel Robson.

References

  1. File TM: Community-acquired pneumonia.

    Lancet 2003, 362:1991-2001. PubMed Abstract | Publisher Full Text OpenURL

  2. File TMJ: Streptococcus pneumoniae and community-acquired pneumonia: a cause for concern.

    Am J Med 2004, 117 Suppl 3A:39S-50S. PubMed Abstract OpenURL

  3. Austrian R: Some aspects of the pneumococcal carrier state.

    J Antimicrob Chemother 1986, 18 Suppl A:35-45. PubMed Abstract OpenURL

  4. Hendley JO, Sande MA, Stewart PM, Gwaltney JMJ: Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types.

    J Infect Dis 1975, 132:55-61. PubMed Abstract OpenURL

  5. Cockeran R, Anderson R, Feldman C: Pneumolysin as a vaccine and drug target in the prevention and treatment of invasive pneumococcal disease.

    Arch Immunol Ther Exp (Warsz ) 2005, 53:189-198. PubMed Abstract OpenURL

  6. Jedrzejas MJ: Pneumococcal virulence factors: structure and function.

    Microbiol Mol Biol Rev 2001, 65:187-207. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  7. Jedrzejas MJ: Extracellular virulence factors of Streptococcus pneumoniae.

    Front Biosci 2004, 9:891-914. PubMed Abstract | Publisher Full Text OpenURL

  8. Polissi A, Pontiggia A, Feger G, Altieri M, Mottl H, Ferrari L, Simon D: Large-scale identification of virulence genes from Streptococcus pneumoniae.

    Infect Immun 1998, 66:5620-5629. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  9. Varon E: [Severe pneumococcal infections: virulence aspects].

    Arch Pediatr 2001, 8 Suppl 4:752s-756s. PubMed Abstract | Publisher Full Text OpenURL

  10. Bogaert D, de Groot R, Hermans PW: Streptococcus pneumoniae colonisation: the key to pneumococcal disease.

    Lancet Infect Dis 2004, 4:144-154. PubMed Abstract | Publisher Full Text OpenURL

  11. Plotkowski MC, Puchelle E, Beck G, Jacquot J, Hannoun C: Adherence of type I Streptococcus pneumoniae to tracheal epithelium of mice infected with influenza A/PR8 virus.

    Am Rev Respir Dis 1986, 134:1040-1044. PubMed Abstract OpenURL

  12. Cundell DR, Weiser JN, Shen J, Young A, Tuomanen EI: Relationship between colonial morphology and adherence of Streptococcus pneumoniae.

    Infect Immun 1995, 63:757-761. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  13. Talbot UM, Paton AW, Paton JC: Uptake of Streptococcus pneumoniae by respiratory epithelial cells.

    Infect Immun 1996, 64:3772-3777. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  14. McCullers JA, Tuomanen EI: Molecular pathogenesis of pneumococcal pneumonia.

    Front Biosci 2001, 6:D877-D889. PubMed Abstract | Publisher Full Text OpenURL

  15. Brown PD, Lerner SA: Community-acquired pneumonia.

    Lancet 1998, 352:1295-1302. PubMed Abstract | Publisher Full Text OpenURL

  16. Novak R, Tuomanen E: Pathogenesis of pneumococcal pneumonia.

    Semin Respir Infect 1999, 14:209-217. PubMed Abstract OpenURL

  17. Cundell DR, Gerard NP, Gerard C, Idanpaan-Heikkila I, Tuomanen EI: Streptococcus pneumoniae anchor to activated human cells by the receptor for platelet-activating factor.

    Nature 1995, 377:435-438. PubMed Abstract | Publisher Full Text OpenURL

  18. Hermoso JA, Lagartera L, Gonzalez A, Stelter M, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M: Insights into pneumococcal pathogenesis from the crystal structure of the modular teichoic acid phosphorylcholine esterase Pce.

    Nat Struct Mol Biol 2005, 12:533-538. PubMed Abstract | Publisher Full Text OpenURL

  19. Shani-Sekler M, Lifshitz S, Hillel I, Dagan R, Grossman N, Fleminger G, Mizrachi-Brauner Y: Initial steps in Streptococcus pneumoniae interaction with and pathogenicity to the host.

    Adv Exp Med Biol 2000, 479:61-71. PubMed Abstract OpenURL

  20. Madsen M, Lebenthal Y, Cheng Q, Smith BL, Hostetter MK: A pneumococcal protein that elicits interleukin-8 from pulmonary epithelial cells.

    J Infect Dis 2000, 181:1330-1336. PubMed Abstract | Publisher Full Text OpenURL

  21. Rosenow C, Ryan P, Weiser JN, Johnson S, Fontan P, Ortqvist A, Masure HR: Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae.

    Mol Microbiol 1997, 25:819-829. PubMed Abstract | Publisher Full Text OpenURL

  22. Smith BL, Hostetter MK: C3 as substrate for adhesion of Streptococcus pneumoniae.

    J Infect Dis 2000, 182:497-508. PubMed Abstract | Publisher Full Text OpenURL

  23. Murdoch C, Read RC, Zhang Q, Finn A: Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells.

    J Infect Dis 2002, 186:1253-1260. PubMed Abstract | Publisher Full Text OpenURL

  24. Strunk RC, Eidlen DM, Mason RJ: Pulmonary alveolar type II epithelial cells synthesize and secrete proteins of the classical and alternative complement pathways.

    J Clin Invest 1988, 81:1419-1426. PubMed Abstract | PubMed Central Full Text OpenURL

  25. Brooks-Walter A, Briles DE, Hollingshead SK: The pspC gene of Streptococcus pneumoniae encodes a polymorphic protein, PspC, which elicits cross-reactive antibodies to PspA and provides immunity to pneumococcal bacteremia.

    Infect Immun 1999, 67:6533-6542. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  26. Lefevre JC, Faucon G, Sicard AM, Gasc AM: DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis.

    J Clin Microbiol 1993, 31:2724-2728. PubMed Abstract | PubMed Central Full Text OpenURL

  27. Arai S, Konda T, Wad A, Matsunaga Y, Okabe N, Watanabe H, Inouye S: Use of antiserum-coated latex particles for serotyping Streptococcus pneumoniae.

    Microbiol Immunol 2001, 45:159-162. PubMed Abstract | Publisher Full Text OpenURL

  28. Briese T, Hakenbeck R: Interaction of the pneumococcal amidase with lipoteichoic acid and choline.

    Eur J Biochem 1985, 146:417-427. PubMed Abstract | Publisher Full Text OpenURL

  29. Cundell DR, Gerard C, Idanpaan-Heikkila I, Tuomanen EI, Gerard NP: PAf receptor anchors Streptococcus pneumoniae to activated human endothelial cells.

    Adv Exp Med Biol 1996, 416:89-94. PubMed Abstract OpenURL

  30. Elm C, Rohde M, Vaerman JP, Chhatwal GS, Hammerschmidt S: Characterization of the interaction of the pneumococcal surface protein SpsA with the human polymeric immunoglobulin receptor (hpIgR).

    Indian J Med Res 2004, 119 Suppl:61-65. PubMed Abstract | Publisher Full Text OpenURL

  31. Centers for Disease Control and Prevention: Streptococcus pneumoniae Disease.

    Available at: http://www cdc gov/ncidod/dbmd/diseaseinfo/streppneum_t htm

    5 AD

    OpenURL

  32. Kelly T, Dillard JP, Yother J: Effect of genetic switching of capsular type on virulence of Streptococcus pneumoniae.

    Infect Immun 1994, 62:1813-1819. PubMed Abstract | PubMed Central Full Text OpenURL

  33. Martens P, Worm SW, Lundgren B, Konradsen HB, Benfield T: Serotype-specific mortality from invasive Streptococcus pneumoniae disease revisited.

    BMC Infect Dis 2004, 4:21. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  34. Zhang JR, Mostov KE, Lamm ME, Nanno M, Shimida S, Ohwaki M, Tuomanen E: The polymeric immunoglobulin receptor translocates pneumococci across human nasopharyngeal epithelial cells.

    Cell 2000, 102:827-837. PubMed Abstract | Publisher Full Text OpenURL

  35. Orihuela CJ, Gao G, Francis KP, Yu J, Tuomanen EI: Tissue-specific contributions of pneumococcal virulence factors to pathogenesis.

    J Infect Dis 2004, 190:1661-1669. PubMed Abstract | Publisher Full Text OpenURL

  36. Berry AM, Paton JC: Additive attenuation of virulence of Streptococcus pneumoniae by mutation of the genes encoding pneumolysin and other putative pneumococcal virulence proteins.

    Infect Immun 2000, 68:133-140. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  37. Weiser JN, Austrian R, Sreenivasan PK, Masure HR: Phase variation in pneumococcal opacity: relationship between colonial morphology and nasopharyngeal colonization.

    Infect Immun 1994, 62:2582-2589. PubMed Abstract | PubMed Central Full Text OpenURL

  38. Kim JO, Weiser JN: Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptococcus pneumoniae.

    J Infect Dis 1998, 177:368-377. PubMed Abstract OpenURL

  39. McCool TL, Cate TR, Tuomanen EI, Adrian P, Mitchell TJ, Weiser JN: Serum immunoglobulin G response to candidate vaccine antigens during experimental human pneumococcal colonization.

    Infect Immun 2003, 71:5724-5732. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  40. Ogunniyi AD, Woodrow MC, Poolman JT, Paton JC: Protection against Streptococcus pneumoniae elicited by immunization with pneumolysin and CbpA.

    Infect Immun 2001, 69:5997-6003. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  41. Zhang Q, Bernatoniene J, Bagrade L, Pollard AJ, Mitchell TJ, Paton JC, Finn A: Serum and mucosal antibody responses to pneumococcal protein antigens in children:relationships with carriage status.

    Eur J Immunol 2006, 36:46-57. PubMed Abstract | Publisher Full Text OpenURL

  42. Jones MR, Simms BT, Lupa MM, Kogan MS, Mizgerd JP: Lung NF-kappaB activation and neutrophil recruitment require IL-1 and TNF receptor signaling during pneumococcal pneumonia.

    J Immunol 2005, 175:7530-7535. PubMed Abstract | Publisher Full Text OpenURL

  43. Sadikot RT, Han W, Everhart MB, Zoia O, Peebles RS, Jansen ED, Yull FE, Christman JW, Blackwell TS: Selective I kappa B kinase expression in airway epithelium generates neutrophilic lung inflammation.

    J Immunol 2003, 170:1091-1098. PubMed Abstract | Publisher Full Text OpenURL

  44. Stockley RA: Role of inflammation in respiratory tract infections.

    Am J Med 1995, 99:8S-13S. PubMed Abstract | Publisher Full Text OpenURL

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1471-2334/6/71/prepub