LightCycler™ amplification profile of the TPR assay with Bordetella isolates. The samples correspond to: 1–8 = dilution series of B. pertussis ATCC 9797 (108cfu/ml to 101cfu/ml, respectively), 9 = B. bronchiseptica ATCC 10580, 10 = B. bronchiseptica ATCC 29513, 11 = B. parapertussis ATCC 1533, 12 = B. parapertussis 33047, 13 = B. holmesii ATCC 51541, 14 = B. holmseii 98P1995. The amplified product is 167 bp and the agarose gel of products is given as an inlay (numbers corresponding to samples in the amplification curves). Stocks of Bordetella isolates were approximately 106cfu/ml unless otherwise quoted.
Knorr et al. BMC Infectious Diseases 2006 6:62 doi:10.1186/1471-2334-6-62