Figure 2.

LightCyclerâ„¢ amplification profile of the IS481 HP probe assay with a dilution series of B. pertussis ATCC 9797 strain of B. pertussis was utilized (106 cfu/ml to 10-3 cfu/ml). Analytical sensitivity of this assay is 10 cfu/ml (0.1 cfu input). The dashed lines correspond to the average Ct value where conventional results are: 1 = DFA positive and culture positive, 2 = DFA negative, culture positive, 3 = DFA negative, culture negative. HP = hybridization probe, Ct = crossing threshold, DFA = direct fluorescent antigen

Knorr et al. BMC Infectious Diseases 2006 6:62   doi:10.1186/1471-2334-6-62
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