Alignment based on B. pertussis ATCC 9797 with primers and probe for real-time PCR indicated. The selected forward primer is labeled box 1 (base pairs 51–69) the reverse primer as box 2 (base pairs 218–199) and the probe by box 3 (base pairs 73–89). GenBank accession numbers for each isolate as listed in order above are AF157344, AF157363, AF157362, AF157364, AF157347, AF157357, AF157361, respectively.
Knorr et al. BMC Infectious Diseases 2006 6:62 doi:10.1186/1471-2334-6-62