Correction

Correction: Detection of Chlamydia in the peripheral blood cells of normal donors using in vitro culture, immunofluorescence microscopy and flow cytometry techniques

Frances Cirino¹ , Wilmore C Webley¹ , Corrie West¹ , Nancy L Croteau¹ , Chester Andrzejewski Jr² and Elizabeth S Stuart¹

¹  Department of Microbiology, University of Massachusetts, Amherst Massachusetts, 01003, USA

²  Department of Transfusion Medicine Baystate Medical Center, Springfield, Massachusetts, 01199, USA

author email corresponding author email

BMC Infectious Diseases 2006, 6:165doi:10.1186/1471-2334-6-165

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2334/6/165

Received:	13 November 2006
Accepted:	16 November 2006
Published:	16 November 2006

© 2006 Cirino et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Correction

Upon close examination of the representative image depicted in 'figure 2' from [1] and a figure published elsewhere [2], we realized that the 'figure 2' of this work was a lower magnification of a section of the same microscope slide used in the previous manuscript [2]. The experiments leading to the data for these two manuscripts were generated during the same time period, in the same laboratory and were compiled, annotated and stored in the same folder, since pictures are taken in bulk at our microscopy facility on a fee for service basis. The figures both describe tissue culture of Chlamydia from clinical samples on the same tissue culture substrate (J774A.1 cells) and were in a single data base of figures with different alpha numeric designations.

We believe that sometime during the compilation and annotation process, the representative 'figure 2' previously shown in this work was incorrectly labeled. After careful scrutiny, we have determined that the image was in fact taken from a culture coverslip that was infected with lysates from pediatric bronchial lavage samples and not normal blood donor buffy coat (BC) samples. The original published figure was a 600× immunofluorescence image [2], part of which can be seen in the lower magnification image (400×) in this work.

The authors deeply regret this error in image annotation and later image selection and therefore submit a representative image from this work to replace the earlier incorrect image depicted in 'figure 2', which is provided with this manuscript (see figure 1).

References

1. Cirino F, Webley WC, West C, Croteau NL, Andrzejewski C Jr., Stuart ES: Detection of Chlamydia in the peripheral blood cells of normal donors using in vitro culture, immunofluorescence microscopy and Flow Cytometry techniques.

   BMC Infect Dis 2006, 6:23. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text

2. Webley WC, Salva PS, Andrzejewski C, Cirino F, West CA, Tilahun Y, Stuart ES: The bronchial lavage of pediatric patients with asthma contains infectious Chlamydia.

   Am J Respir Crit Care Med 2005, 171(10):1083-1088. PubMed Abstract | Publisher Full Text

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1471-2334/6/165/prepub

Have something to say? Post a comment on this article!