HIV-1 BaL inactivation in seminal plasma-Aquateric formulation mixtures. Increasing volumes of seminal plasma were added to aliquots of formulation 2 (see Methods section). After 5 min at 37°C, the samples were cooled in ice, and residual infectivity was measured. The decrease in extent of virus inactivation reflects both dilutions of the Aquateric formulation by seminal plasma and a concomitant pH increase. Samples diluted ≤ 8-fold were cytotoxic due to low pH, and the percentage of virus inactivation could not be determined.
Neurath et al. BMC Infectious Diseases 2006 6:150 doi:10.1186/1471-2334-6-150