Table 1

Decimal reduction times (D-values) for the bacteria in different chemical agent solutions.

Bacteria

Disinfectant concentration

Survivors1

D value

2t = n × D

2t = n × D

(%)

(mg/L)

logN

(min)

3n = 6-log10

n = 12-log10

(min)

(min)


    SODIUM DICHLORO ISOCYANURATE
(pH = 7.0)

A. calcoaceticus

0.1

1000

-0.17t

5.9

35.4

70.8

E. cloacae

0.1

1000

-0.21t

4.7

28.2

56.4

E. coli

0.1

1000

-0.17t

5.9

35.4

70.8

S. aureus

0.1

1000

-0.20t

5.0

30.0

60

S. marcescens

0.1

1000

-0.23t

4.3

25.8

51.6

B. stearothermophilus

0.2

2000

-0.22t

4.4

26.6

53.3

B. subtilis

0.2

2000

-0.26t

3.8

22.5

45

    SODIUM HYPOCHLORITE
(pH = 7.0)

E. coli

0.05

500

-0.16t

6.1

36.4

72.9

B. stearothermophilus

0.05

500

-0.106t

9.4

56.4

112.8

B. subtilis

0.05

500

-0.097t

10.3

61.8

123.6

B. stearothermophilus

0.1

1000

-0.28t

3.5

20.9

41.8

B. subtilis

0.1

1000

-0.31t

3.2

19.2

38.3

    SODIUM HYPOCHLORITE
(pH = 7.0)

A. calcoaceticus

0.025

250

-0.16t

6.2

37.3

74.6

E. cloacae

0.025

250

-0.13t

7.5

44.8

89.6

E. coli

0.025

250

-0.13t

7.5

45.1

90.1

S. aureus

0.025

250

-0.21t

4.7

27.9

55.8

S. marcescens

0.025

250

-0.149t

6.7

40.1

80.2

B. stearothermophilus

0.025

250

-0.041t

24.0

144.0

288.0

B. subtilis

0.025

250

-0.048t

20.6

123.6

247.2

    GLUTARALDEHYDE
(pH = 7.4)

A. calcoaceticus

2.0

20000

-0.21t

4.7

28.2

56.4

E. cloacae

2.0

20000

-0.15t

6.7

40.2

80.4

E. coli

2.0

20000

-0.14t

7.1

42.6

85.2

S. aureus

2.0

20000

-0.17t

5.9

35.4

70.8

S. marcescens

2.0

20000

-0.20t

5.0

30.0

60.0

B. stearothermophilus

2.0

20000

-0.04t

25.0

150.0

300.0

B. subtilis

2.0

20000

-0.04t

25.0

150.0

300.0

    FORMALDEHYDE
(pH = 6.5)

A. calcoaceticus

0.5

5000

-0.19t

5.2

31.2

62.4

E. cloacae

0.5

5000

-0.22t

4.5

27.0

54

S. marcescens

0.5

5000

-0.48t

2.1

12.6

25.2

B. stearothermophilus

0.5

5000

-0.10t

10.9

65.4

130.8

B. subtilis

0.5

5000

-0.13t

11.8

70.8

141.6

    CHLORHEXIDINE
(pH = 6.2)

A. calcoaceticus

0.4

4000

-0.24t

4.1

24.6

49.2

E. cloacae

0.4

4000

-0.12t

8.3

49.8

99.6

E. coli

0.4

4000

-0.34t

3.0

18.0

36.0

S. aureus

0.4

4000

-0.17t

5.9

35.4

70.8

S. marcescens

0.4

4000

-0.25t

4.0

24.0

48.0

B. stearothermophilus

2.0

20000

-0.11t

9.1

54.6

109.2

B. subtilis

2.0

20000

-0.15t

6.7

40.2

80.4

    MINNCARE
5 (pH = 2.3)

A. calcoaceticus

1.0

10000

-0.30t

3.4

20.4

40.8

E. cloacae

1.0

10000

-0.29t

3.5

21.0

42.0

E. coli

1.0

10000

-0.15t

6.7

40.2

80.4

S. aureus

1.0

10000

-0.25t

4.0

24.0

48.0

S. marcescens

1.0

10000

-0.28t

3.6

21.6

43.2

B. stearothermophilus

1.0

10000

-0.11t

9.1

54.6

109.2

B. subtilis

1.0

10000

-0.17t

5.9

35.4

70.8

    HYDROGEN PEROXIDE
(pH = 3.3)

E. cloacae

1.5

15000

-0.56t

1.8

10.8

21.6

E. coli

1.5

15000

-0.31t

3.2

19.2

38.4

S. aureus

1.5

15000

-0.30t

3.4

20.4

40.8

B. stearothermophilus

26.5

265000

-0.21t

4.7

28.2

56.4

B. subtilis

1.5

15000

-0.02t

55.2

331.2

662.4


1 Survivor Curve: log Nf = log No-1/D × t; No= bioburden; Nf= survival population; D-value = decimal reduction time; (-1/D) = slope. 2 t = n × D, where: t = total exposure time (min); n = log10 reduced cycles 3 t = n*D and n = 6-log10, t = the exposure time for a 6-log10 reduction in the bioburden (No) with a defined D-value4 t = n*D and n = 12-log10, t = the exposure time for a 12-log10 reduction in the bioburden (No) with a defined D-value 5MINNCARE = 0.45 % peracetic acid + 2.2 % hydrogen peroxide

Mazzola et al. BMC Infectious Diseases 2003 3:24   doi:10.1186/1471-2334-3-24

Open Data