The differential expression of virulence genes is often used by microbial pathogens in adapting to the environment of their host. The differential expression of such sets of genes can be regulated by RNA polymerase sigma factors. Some sigma factors are differentially expressed, which can provide a means to identifying other differentially expressed genes such as those whose expression are controlled by the sigma factor.
To identify sigma factor-regulated genes, we developed a method, termed I-TRAP, for the
To illustrate the development and use of the I-TRAP approach, the construction of the vectors, host strains, and library necessary to identify SigmaE-regulated genes of Mycobacterium tuberculosis is described.
The I-TRAP method should be a versatile and useful method for identifying and characterizing promoter activity under a variety of conditions and in response to various regulatory proteins. In our study, we isolated 360 clones that may contain plasmids carrying SigmaE-regulated promoters genes of M. tuberculosis.
The ability of Mycobacterium tuberculosis bacteria to infect and replicate inside host mononuclear phagocytes is key to its ability to establish an infection and cause tuberculosis. To elucidate the mechanisms used by tubercle bacilli to survive in macrophages, researchers at several laboratories are studying mycobacterial genes specifically expressed during growth in macrophages [1-6]. Among the genes specifically expressed during intracellular growth are genes encoding two extracytoplasmic function sigma factors, SigE (σE) and SigH (σH) [3-5]. Sigma factors play critical roles in the recognition of promoters by RNA polymerase. In several systems, individual sigma factors are known to regulate the differential expression of sets of genes involved in specific processes, such as genes involved in the heat-shock response or those required for sporulation in Bacillus subtilis [7,8].
Identifying a differentially expressed sigma factor can provide a means to identifying other differentially expressed genes – genes whose expression are controlled by the sigma factor. To identify sigma factor-regulated genes, we developed a method, termed I-TRAP, for the
Bacterial strains and growth condition
Bacterial strains are listed in Table 1. Escherichia coli strains were grown in Luria Broth (LB) or on LB agar. Mycobacterium smegmatis LR222 strains were grown in Middlebrook 7H9 broth supplemented with 10% albumin-dextrose-catalase (ADC, v/v, Difco Laboratories, Detroit, MI) and 0.05% Tween 80 (v/v, Sigma, St. Louis, MO) at 37°C, with agitation on a rotating platform (100 rpm), or were grown on trypticase soy agar (TSA) plates. M. tuberculosis H37Rv TMC102 cultures were grown in Middlebrook 7H9 broth supplemented with ADC and 0.05% Tween 80 at 37°C in 250 ml nephelometer flasks on a rotating platform (60 rpm) or were grown on Middlebrook 7H10 plates with 10% oleic acid-albumin-dextrose-catalase (OADC, Difco). For selection of E. coli bacteria, drugs were incorporated into the medium at the following concentrations: hygromycin (Invitrogen, Carlsbad, CA), 200 μg/m; kanamycin (Sigma), 25 μg/ml; zeocin (Invitrogen), 25 μg/ml; and gentamicin (Invitrogen), 20 μg/ml. For selection of M. smegmatis bacteria, drugs were incorporated into medium at the following concentrations: hygromycin (Invitrogen), 50 μg/ml; kanamycin (Sigma), 50 μg/ml; zeocin (Invitrogen), 50 μg/ml; and gentamicin (Invitrogen), 5 μg/ml.
Table 1. Bacterial strains and plasmids. Plasmid pAWS2 was provided by Rosalind Van Landingham, pBluescript-int was provided by Bonnie Plikaytis, and pUS267 was provided by Jeremy Dale
Plasmids used in this study, along with their key features and sources, are listed in Table 1. Two of the source plasmids, pCL8 and pAWS2, were constructed as part of unpublished studies. pCL8 is a derivative of pCL5  and contains the luciferase (luc) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene (aph) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the PrpsL-xylE cassette from pTKmx97 in which the BamHI site between the M. smegmatis rpsL promoter and the xylE gene in pTKmx97  was changed into a KpnI site by site-directed PCR mutagenesis. pAWS.2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the EcoRI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).
Construction of pSEint.2
1) The ble gene was PCR amplified from pPICZalphaC as a 375-bp fragment using primers Ble-N and Ble-C; the amplicon was blunt-ended and ligated into the blunt-cloning site of pT7Blue to generate pT7Blue-bleA. 2) The ble gene was recovered as a 382-bp BamHI fragment from pT7Blue-bleA and cloned into BamHI cleaved pMV261 to generate pMV261-ble. 3) A 1077-bp Phsp60-ble cassette was PCR amplified from pMV261-ble using primers 981816 and 981819 and blunt cloned into pT7Blue to generate pT7Blue-bleB. 4) The Phsp60-ble cassette was recovered from pT7Blue-bleB by XbaI digest and cloned into the XbaI site of a pBluescript KS+ based plasmid that had the mycobacteriophage L5 integrase gene cloned into the EcoRI site to generate pSEint.1. 5) The M. tuberculosis sigE gene was PCR amplified from H37Rv genomic DNA using primer 987917 and primer 987916 (which contains a BamHI site), digested with BamHI, and cloned into pMV261 digested with HpaI and BamHI. 6) The Phsp60-sigE cassette was PCR amplified using primers 981816 and 981819 and cloned into pT7Blue to generate pT7Blue-Phsp60-sigE. 7) The Phsp60-sigE cassette was recovered from pT7Blue-Phsp60-sigE by digestion KpnI and cloned into the KpnI site of pSEint.1 to generate pSEint.2.
Construction of pSEKO.4
1) A 757-bp fragment containing 246 bp of the 3' end of the M. smegmatis sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a HpaI restriction site) and primer 7209 and cloned into the EcoRV blunt-cloning site of pT7Blue to generate pSEKO.1. 2) A 760-bp fragment containing 526 bp of the 5' region of the M. smegmatis sigE gene and 234 bp of the region upstream was PCR amplified using primer 7206 and primer 7207 (which contains a HpaI restriction site), digested with HpaI, and cloned into the HpaI site of pSEKO.1 to generate pSEKO.2. These steps resulted in a 2-bp deletion of the sigE gene. 3) The gentamicin-resistance gene (aacC1) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain HpaI restriction sites), digested with HpaI, and cloned into the HpaI site located between the two fragments of the sigE gene in pSEKO.2 to generate pSEKO3. 4) The rpsL promoter was isolated from pCL8 as a BamHI-KpnI fragment and cloned into BamHI-KpnI digested pAWS2 upstream of the lacZ gene. 5) The PrpsL-lacZ cassette was removed from this recombinant by BamHI and EcoRI digestion and cloned into BamHI and EcoRI digested pBluescript SK+. 6) The PrpsL-lacZ cassette was isolated from this subclone as a HindIII fragment, and the ends of the fragment were blunt-ended using the Perfectly Blunt Cloning Kit (Novagen, Madison, WI) and cloned into the ScaI site of pSEKO.3 to generate pSEKO.4.
Construction of pSE.4
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918. Primers 1916 and 1917 have 20 nucleotides of complementary sequence so that the two PCR products can anneal. The two PCR products were mixed, allowed to anneal, and amplified using two primers, one (1915) complementary to the 5' end of the aph gene and a second (1918) complementary to the 3' end of xylE to produce a 1785-bp fragment. The amplified annealed fragment was gel purified and cloned into the SmaI site of pUC19 to generate pSE.1. 2) The hygromycin B-resistance gene (hyg) was recovered from pUS267 by HindIII digestion and cloned into the HindIII site of pSE.1 to generate pSE.2. 3) A 1903-bp fragment containing oriM was recovered from pMV261 by digestion with NotI and MluI, gel purified, blunt ended using the Perfectly Blunt Cloning Kit (Novagen), and ligated into pSE.2 digested with SspI to generate pSE.3. 4) An amplicon containing the E. coli rRNA transcriptional terminator was PCR amplified from pCL5 using primers 5077 and 5078 (which contain XbaI sites), digested with XbaI, and ligated into XbaI digested pSE.3 to generate pSE.4. Function of the terminator was determined by patching hygromycin B-resistant transformants LB medium containing kanamycin.
DNA manipulations and DNA sequencing
Restriction enzyme reactions were performed as recommended by the manufacturer (Invitrogen). Wizard Plus Minipreps DNA Purification System (Promega, Madison, WI) was used to isolate plasmid DNA from E. coli bacteria and from M. smegmatis bacteria as previously described . Phosphatase reactions using Calf Intestinal Alkaline Phosphatase (Invitrogen) and ligation reactions using T4 DNA Ligase (Invitrogen) were carried out according to manufacturer's directions. Sequencing reactions were carried out using the Big Dye Terminator Cycle Sequencing Ready Reaction Kit as recommended by manufacturer (Applied Biosystems). Sequence reactions were analyzed using an ABI 373 DNA sequencer and sequence data were assembled using SeqEd 675 DNA Sequence Editor, version 1.0.3 (Applied Biosystems).
Genomic DNA isolation
M. smegmatis genomic DNA was purified by a modification of the glass-bead lysis method as previously described . M. tuberculosis genomic DNA was isolated as previously described . DNA concentration was estimated using a GeneQuant II apparatus (Amersham Biosciences).
Polymerase chain reaction
The primers used in this study are listed in Table 2. Primers were synthesized on a 381A DNA synthesizer (Applied Biosystems, Foster City, CA) at the Biotechnology Core Facility, National Center for Infectious Diseases, Centers for Disease Control and Prevention. 50 μl amplification reactions contained 100–200 ng DNA template and 45 μl of a reaction mix (200 μM each, deoxynucleotide triphosphates; 1.0 μM each primer; 1.25 U Taq polymerase; 10 mM Tris hydrochloride (pH 8.3); 50 mM KCl; 1.5 mM MgCl2; 0.01% gelatin). Samples were amplified for 30 cycles in a GeneAmp PCR system 2400 thermocycler (Applied Biosystems) with a three-step cycle of denaturation for 30 s at 94°C, annealing for 30 s at 55°C to 70°C (optimized for each primer pair), and extension for 1 min at 72°C. Amplification products were analyzed by electrophoresis through 0.8 to 2.0% agarose-TBE gel and visualized by ethidium-bromide staining.
Table 2. Oligonucleotide primers. Underlined bases are restriction sites relevant to the construction. MS: Mycobacterium smegmatis; MTB: Mycobacterium tuberculosis; Phsp60: promoter for the M. tuberculosis hsp60 gene. PrpsL: promoter for the M. smegmatis rpsL gene
Selection of active promoters
Electrocompetent M. smegmatis LR222::SEint.2 bacteria were electroporated with 5 μg of the library plasmid DNA, and recipients were plated on TSA with kanamycin (50 μg/ml). After incubation at 37°C for 3 days, colonies were pooled and collected by flooding plates with 2 ml Middlebrook 7H9 broth and scraping the colonies off the plate. Plasmid DNA was harvested from the transformants, ethanol precipitated, and resuspended in water.
Screening to identify σE-dependent promoters
Electrocompetent M. smegmatis LR222ΔsigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml). After incubation at 37°C for 3 days, colonies were chilled for 2 h at 4°C. Chilled colonies were sprayed with 0.5 M catechol (Sigma) in 0.05 mM potassium phosphate buffer pH 7.4.
The basic I-TRAP approach (Figure 1) uses a genomic DNA library in a plasmid vector containing a promoterless 'operon' with two reporter genes. One reporter gene, the aph gene which confers resistance to kanamycin, allows for selection of active promoters and a second reporter gene, the xylE gene which encodes catechol 2,3 dioxygenase, allows for screening for loss of promoter activity. In the first step of the I-TRAP approach, the recombinant plasmid library is transformed into bacteria expressing the transcriptional regulator. Transformants containing plasmids with active promoters are isolated by selection on kanamycin-containing media. A subset of the active promoters will be those whose expression depends on the transcriptional regulator. To differentiate between promoters dependent on the regulator and other promoters, plasmid DNA from a pool of the kanamycin-resistant transformants is transformed into bacteria that do not express the transcriptional regulator. The hygromycin B-resistant transformants are screened for XylE expression by spraying colonies with catechol. Yellow colonies arise from bacteria that contain a plasmid with a transcriptionally active promoter. White colonies arise from bacteria that contain a plasmid with a promoter that is inactive in the absence of the transcriptional regulator.
Figure 1. Schematic of I-TRAP approach.
To develop the I-TRAP method for identifying M. tuberculosis promoters dependent on the expression of σE, a strain of M. smegmatis over-expressing the M. tuberculosis σE, a σE mutant strain of M. smegmatis, and a M. tuberculosis promoter-trap library were created.
Construction of σE-over-expressing strain of M. smegmatis
The σE-over-expressing strain of M. smegmatis was made by transforming M. smegmatis LR222 bacteria with pSEint.2 (Figure 2). The pSEint.2 plasmid contains an oriE for plasmid replication in E. coli, the mycobacteriophage L5 integrase and attP site to allow integration into the M. smegmatis chromosome, two selectable genes, ble (bleomycin or zeocin resistance) and amp (ampicillin resistance), and the M. tuberculosis RV1221 ORF cloned downstream of the M. tuberculosis hsp60 promoter. In this strain, the M. tuberculosis sigE gene is maintained in a single copy in the genome and is constitutively expressed from the hsp60 promoter. This strain is designated M. smegmatis LR222::SEint.2 (Table 1).
Figure 2. Map of pSEint.2. pSEint.2 contains an oriE for plasmid replication in E. coli, the mycobacteriophage L5 integrase (int) and attP to allow integration into the M. smegmatis chromosome, a ble gene (bleomycin resistance) for selection in mycobacteria, an amp gene (ampicillin resistance) for selection in E. coli, and the M. tuberculosis sigE gene under the control of the constitutively expressed hsp60 promoter. Arrowheads indicate direction of transcription.
Construction of sigE mutant strain of M. smegmatis
The suicide plasmid pSEKO.4 (Figure 3) was constructed to interrupt the M. smegmatis sigE gene by gene replacement . pSEKO.4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene and the lacZ gene. The pSEKO.4 DNA (1 μg) was UV-irradiated in a UV Stratalinker 1800 (Stratagene) at 100 mJ cm-2 and electroporated into M. smegmatis cells. Following outgrowth at 37°C for 4 h, transformants were recovered by plating on TSA containing gentamicin and X-Gal. 24 white transformants were analyzed by PCR using primers (7997 and 7998) that bind to the sigE gene on either side of the aacC1 gene (Table 2). Of the 24 transformants, 11 had the M. smegmatis sigE gene disrupted by aacC1, 9 had only a single recombination event, and 4 only had a wild-type copy of sigE (data not shown). One transformant was chosen as the M. smegmatis sigE mutant strain and designated LR222Δ sigE (Table 1).
Figure 3. Map of pSEKO.4 pSEKO.4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene, aac1, the lacZ gene for use as a screen against single recombination events, and an oriE for plasmid replication in E. coli. Arrowheads indicate direction of transcription. The construction of the mutated sigE gene resulted in an insertion of the aac1 cassette and a 2-bp deletion of the sigE gene at the site of insertion.
Construction of a library in the promoter-trap plasmid
pSE.4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis, an oriE for plasmid replication in E. coli, and a hyg gene for selection in E. coli and M. smegmatis bacteria. The plasmid contains a unique BamHI site upstream of the promoterless reporter operon for cloning of potential promoters. To generate fragments of the M. tuberculosis H37Rv genome, ~3.0 μg of genomic DNA was digested with 1 U Sau3AI in 20 μl total volume for 2 min at 37°C and the reaction stopped by heating at 65°C for 10 min. This generated fragments between 100 and ~6,000 bp (data not shown). Sau3AI was removed from the reaction by phenol-chloroform extraction. DNA was ethanol precipitated and resuspended in 20 μl Tris buffer pH 7.4. Approximately 470 ng digested genomic DNA was ligated with ~200 ng pSE.4 DNA that had been digested with BamHI and phosphatased. DNA was ethanol precipitated from the ligation reaction and resuspended in water; 200 ng DNA was electroporated into Electromax DH5α cells. Recipient bacteria were spread on LB agar containing hygromycin B. After overnight incubation at 37°C, 50 colonies were selected at random, plasmid DNA was harvested, and genomic DNA inserts were sequenced using primer TN5-9R (Table 2). All plasmids contained a unique M. tuberculosis DNA segment and had an average insert size of approximately 200 bp (data not shown).
Figure 4. Map of pSE.4. pSE.4 is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator cloned upstream of the promoterless aph and xylE genes, a unique BamHI cloning site, an oriM for plasmid replication in mycobacteria, an oriE for plasmid replication in E. coli, and a hyg gene for selection in E. coli and M. smegmatis bacteria. Arrowheads indicate direction of transcription.
To generate a library of recombinant plasmids, 200 ng of the ligated sample were electroporated into Electromax DH5α cells. The entire mixture was plated on 117 150 mm LB agar plates containing hygromycin B. About 117,000 transformants were collected and pooled by flooding each plate with 2 ml LB and scraping the colonies off the plate. Plasmid DNA was harvested from the pooled E. coli transformants, ethanol precipitated, and resuspended in water.
Promoter selection and scoring
To recover plasmids containing active promoters, the plasmid library DNA was electroporated into M. smegmatis LR222::SEint.2 bacteria, which constitutively express the M. tuberculosis sigE gene. To determine the proportion of active promoters in the library, a portion of the transformation mixture was spread on medium containing hygromycin B (all recipients), and a portion was spread on medium containing kanamycin (recipients with an active promoter). 1.93 × 106 hygromycin B-resistant colony forming units (CFUs) /ml and 3.75 × 104 kanamycin-resistant CFU/ml were recovered; therefore, approximately 1.9% of transformants contained an active promoter fragment. When the initial pool of kanamycin-resistant colonies sprayed with catechol, the colonies were light to dark yellow indicating a wide range of XylE activity.
To differentiate between σE-dependent and -independent promoters, approximately 40,000 kanamycin-resistant transformants were pooled, and plasmid DNA was isolated and electroporated into M. smegmatis LR222::Δ sigE bacteria, in which the wild type copy of the sigE gene had been disrupted with the gentamicin-resistance gene. Transformants were plated on hygromycin B and assayed for catechol 2,3 dioxygenase activity by spraying colonies with 0.5 M catechol. About 20,000 colonies were sprayed with catechol, and a range of color intensity from white to bright yellow was observed. A total of 360 white and light yellow colonies were recovered. Overall, about 1.8% of hygromycin B-resistant colonies were white or light yellow.
To confirm the phenotypes of plasmids recovered in this two-step procedure, plasmids were recovered from 18 white colonies and individually transformed into M. smegmatis LR222::SEint.2 (SigE-expressing) and M. smegmatis LR222::Δ sigE bacteria. All 18 plasmids generated only white colonies when transformed into the σE-mutant strain. However, although all 18 plasmids generated yellow colonies when transformed into σE-expressing bacteria, some plasmids produced a mixture of white and yellow colonies. These white colonies were consistently white on retesting and their plasmids usually contained deletions that removed the cloned M. tuberculosis sequence. Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.
A preliminary analysis of the sequences of the inserts in the 18 clones identified 6 inserts that had matches with the SigE consensus sequence . Interestingly, none of these genes were identified as being SigE-induced genes in microarray studies , although the expression of one of the genes, Rv3223c (sigH), is thought to be SigE-regulated .
A key feature of the I-TRAP method is that it allows two degrees of promoter analysis, i.e., with and without a transcriptional regulator. The first step allows selection of all active promoters under one condition, and the second step sorts promoters by activity dependent on a second condition. In the study reported here, we used this method to identify potential σE-induced genes. The basic I-TRAP approach might also be used to identify promoters repressed by a particular protein. The transformation steps are reversed so that the promoters are selected in the mutant background first (i.e., no repressor) and then screened for loss of activity in the presence of the repressor protein.
The I-TRAP method might also be used to characterize promoter activity in two environmental conditions in a manner similar to the IVET approach . This is useful because, in some cases, the factor necessary for transcriptional activation during a particular environmental condition is unknown. In this case, after exposing bacteria containing the recombinant library to an environmental condition (e.g., a heat shock or acid shock or growth in medium A), antibiotics could be added to the medium to kill any bacteria that had not expressed the antibiotic resistance reporter gene during the exposure. For mycobacteria, a two-hour treatment with kanamycin would be sufficient to kill bacteria that had not expressed the kanamycin-resistance gene during the stress. The resistant bacteria would then be grown in a second medium or in the absence of a stress, and colonies scored for lack of promoter activity in the second condition by expression of the xylE reporter gene. White colonies would contain a plasmid with a promoter that is active only during the first condition. Using I-TRAP for this purpose may provide a means to identify promoters dependent on any of several regulatory proteins that may be active during the stress and to obtain a global overview of gene expression during the stress.
In the I-TRAP method, the recipient strain can be the native bacterium or a surrogate host. We used M. smegmatis as the host because it grows much more rapidly than does M. tuberculosis (generation time of 3 hrs vs. 24 hrs), does not require BSL-3 facilities, and is more easily manipulated genetically than M. tuberculosis. One possible advantage of using a surrogate host is that studies could be focused on one particular regulator which might avoid complications due to other regulatory proteins in the cell that may be able to recognize promoters in the library. Of course, the surrogate host must not have a regulatory protein that recognizes the same promoters as the regulator being studied. In our studies, the M. smegmatis σE protein has 92% homology with the M. tuberculosis σE protein . While this suggests that the M. tuberculosis σE protein should be able to interact properly with the M. smegmatis RNA polymerase, it also suggests that the M. smegmatis σE homolog might be able to recognize M. tuberculosis σE-dependent promoters. Because of this, we constructed an M. smegmatis host strain that lacked any functional σE protein.
An assumption of the I-TRAP approach is that the sequences cloned in the plasmids will be recognized by transcriptional regulatory proteins in the same manner as the intact sequences in the genome. The cloning process might generate false-positive or false-negative results because of the cloned sequences being recognized out of the context of the surrounding genomic sequences.
Another assumption of the I-TRAP approach is that over-expression of a transcriptional regulator will be sufficient to induce gene expression from its regulated promoters. This suggests that proteins, such as PhoP, that require activation to promote transcription may not be approachable with this method unless the over-expressed regulatory protein can also be activated. Also, certain regulatory proteins may not be suitable for study in a surrogate host bacterium . For proteins that require the presence of additional factors, such as σN, or interaction with host proteins to be active, such as σE with RNA polymerase, use of the native bacterium or closely related surrogate host may be required to ensure the presence of the necessary interacting proteins . Some of these additional factors, however, may only be expressed or active under a particular growth condition and therefore may also need to be conditionally expressed along with the transcription factor. Promoters that require both a positive transcription regulator and the absence of a repressor protein may be missed because expressing the positive transcription factor alone may not be sufficient to induce transcription at such promoters. Another limitation of the I-TRAP method is that some transcription factors may not be stably maintained in the bacterium or may be lethal to the bacterium when over-expressed. To avoid this problem, an inducible promoter could be used to promote transcription of the gene encoding the transcription factor.
Another possible complication of this approach is that expressing a foreign protein may turn on the stress response of the host bacterium. Transcription factors mediating the stress response to the expressed foreign protein may be able to recognize promoters within the library and promote transcription of the reporter genes, thus producing the false positives in the two-step screen.
Because the first step of the I-TRAP method involves selection for active promoters, one could easily begin with a library containing recombinants representing all possible promoters in a bacterial genome. Unfortunately, the recombinant DNA library used in this study contained only ~65% of the genome in the correct orientation upstream of the reporter genes, which will limit the number of different promoters we can recover using this library. For the screening step, we used the xylE gene because catechol 2,3 dioxygenase activity is easily measured by spraying colonies with catechol and the amount of color produced is a rough measure of promoter activity . In our screen, white, pale yellow, and bright yellow colonies were observed, indicating a wide range of promoter activity. Pale yellow colonies may represent weak σE-independent promoters or promoters that are recognized by another sigma factor in addition to σE, providing a low level of constitutive expression. These possibilities could be distinguished by transformation of plasmids from individual clones into the σE-over-expressing strain and directly comparing of XylE activity in the presence and absence of σE. Unfortunately, it appears that expression of XylE in M. smegmatis may be detrimental and may lead to plasmid instability and loss of the inserted DNA. This raises the possibility that some white colonies may arise in the second step of I-TRAP by loss of a promoter fragment from a plasmid recovered in the first step. Nonetheless, the I-TRAP method should greatly enrich for promoters with the desired activities. As is generally true for genetic screens, it is essential to study any identified promoters in their native state to understand the regulation of their expression.
The I-TRAP method is a versatile and useful method for characterizing promoter activity under a variety of conditions and in response to various regulatory proteins. In our study, we isolated 360 clones that may contain plasmids carrying SigE-regulated promoters genes of M. tuberculosis. The analysis of these clones will be reported elsewhere.
No competing interests for either author.
The work is a portion of MA's doctoral thesis. MA designed and conducted the studies, analyzed the results, and drafted the manuscript. TS participated in the design and coordination of the study, analysis of results, and preparation of the manuscript. All authors read and approved the final manuscript.
We thank Bonnie Plikaytis for advice and technical assistance and Rosalind Van Landingham, Bonnie Plikaytis, and Jeremy Dale for providing plasmids. We thank the Biotechnology Core Facility of the Scientific Resources Program of the National Center for Infectious Diseases for providing oligonucleotides primers.
Use of trade names is for identification only and does not constitute endorsement by the U.S. Department of Health and Human Services, the Public Health Services, or the Centers for Disease Control and Prevention.
Genes Dev 1989, 3:1462-1471. PubMed Abstract
Ann N Y Acad Sci 1996, 797:32-41. PubMed Abstract
Plikaytis BB, King CH: Nucleic-acid extraction: plasmid/cosmid DNA. In Mycobacterial Protocols. Volume 101. Edited by Parish T, Stoker NG. Totowa, NJ: Humana Press; 1998::45-50. Publisher Full Text
J Clin Microbiol 1990, 28:1913-1917. PubMed Abstract
Meth Enzymol 1991, 204:537-555. PubMed Abstract
Science 1993, 259:686-688. PubMed Abstract
Mol Microbiol 1993, 10:903-909. PubMed Abstract
Antimicrob Agents Chemother 1994, 38:805-811. PubMed Abstract
The pre-publication history for this paper can be accessed here: