Table 2 |
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|
Studies without controls of Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells |
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|
Reference |
Country |
Study group |
Prevalence (%) |
Blood sampling |
DNA Extraction |
PCRa target |
|
|
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|
Blasi, 1999 [30] |
Italy |
Aortic aneurysm |
19 of 41 (46.3) |
8 mL CPTc tube |
Phenol-chloroform |
Nested MOMPd |
|
Blasi, 2000 (abstract) |
Italy |
COPDe |
10 of 21 (47.6) |
8 mL CPT tube |
Not stated |
Not stated |
|
Bodetti 2000 [34] |
Australia |
Blood donors |
10 of 60 (16.7) |
9 mL EDTA |
Not stated |
Nested MOMP |
|
Haranaga, 2001 [36] |
USA |
Blood donors |
21 of 237 (8.9) |
5 mL EDTA |
QIAamp DNA extraction |
Touchdown 16S rDNA |
|
Iliescu, 2000 [33] |
Canada |
Dialysis |
33 of 55 (60.0) |
10 mL heparin |
QIAamp DNA extraction |
53 kDa protein |
|
Maass, 2000 [29] |
Germany |
Unstable angina, myocardial infarction |
65 of 238 (27.3) |
8 mL EDTAb, CD14+ cell selection |
QIAamp DNA extraction |
Nested pstI cloned fragment |
|
Rassu, 2001 [35] |
Italy |
Blood donors |
78 of 169 (46.2) |
8 mL EDTA |
Phenol-chloroform |
Nested MOMP |
|
Smieja, 2002 [32] |
Canada |
COPD |
24 of 100 (24.0) |
8 mL CPT tube |
QIAamp DNA extraction |
Nested MOMP |
|
Taylor-Robinson, 2000 [31] |
United Kingdom |
Peripheral vascular disease |
Not stated (47.0) |
Not stated |
Not stated |
Not stated |
|
|
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|
a PCR, polymerase chain reaction. b EDTA, ethylene diamine tetra-acetic acid c CPT, BD cell preparation tube (BD Vacutainer Systems, Franklin Lakes NJ, USA) d MOMP, major outer membrane protein e COPD, chronic obstructive pulmonary disease |
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|
Smieja et al. BMC Infectious Diseases 2002 2:21 doi:10.1186/1471-2334-2-21 |
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