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This article is part of the supplement: Abstracts from the 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)

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Host factors influencing the activation of tuberculosis in HIV positive individuals with latent TB infection

Kamakshi Prudhula Devalraju, Sharada Ramseri Sunder, Vijaya Lakshmi Valluri* and Arunabala Chowdary

Author Affiliations

LEPRA India – Blue Peter Public Health & Research Centre, Hyderabad, AP, India

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BMC Infectious Diseases 2014, 14(Suppl 3):E16  doi:10.1186/1471-2334-14-S3-E16

The electronic version of this article is the complete one and can be found online at:

Published:27 May 2014

© 2014 Devalraju et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.


TB kills 1.3 million persons annually, including 275,000 people in India. An estimated 40% of the population in India has LTBI (Latent Tuberculosis Infection), a significant percentage of them being infected with HIV. HIV infection increases the likelihood of LTBI progressing to active TB. Vaccination and immunotherapeutic strategies are alternative approaches that contribute greatly to TB control in HIV+ persons with LTBI.


Peripheral blood was drawn from, HIV+ and HIV- individuals with and without LTBI. PBMC and CD14+ monocytes were isolated and 2 million cells were cultured with γ irradiated M. tuberculosis H37Rv, CFP-10. Cultures were terminated after 96 hours, and cells were stained for CD4, CD25, FOXP3 and D4GDI. IFN-γ, IL-17, IL-22 were estimated in the supernatants by ELISA. RNA was isolated from CD14 cells and Realtime PCR was performed to quantitate c-maf expression. P<0.05 was considered statistically significant.


In response to M. tuberculosis, FoxP3+ cells expand in HIV+ and healthy LTBI+ donors. In contrast, D4GDI+FoxP3+ cells expand only in healthy LTBI+ individuals. IL-17 and IL-22 are significantly high in HIV-LTBI+ individuals, increased c-maf expression in monocytes was observed in HIV+ LTBI+ individuals


IL-17 and IL-22 can be helpful to control reactivation of tuberculosis in HIV+ individuals.