Open Access Research article

Inventory study of non-tuberculous mycobacteria in the European Union

Marieke J van der Werf1*, Csaba Ködmön1, Vera Katalinić-Janković2, Tiina Kummik3, Hanna Soini4, Elvira Richter5, Dimitrios Papaventsis6, Enrico Tortoli7, Monique Perrin8, Dick van Soolingen9, Manca Žolnir-Dovč10 and Vibeke Østergaard Thomsen11

Author Affiliations

1 European Centre for Disease Prevention and Control, Stockholm, Sweden

2 National Mycobacterium Reference Laboratory, Croatian National Institute of Public Health, Zagreb, Croatia

3 Department of Mycobacteriology, Tartu University Hospital, Tartu, Estonia

4 National Institute for Health and Welfare, Turku, Finland

5 National Reference Laboratory for Mycobacteria, Forschungszentrum Borstel, Borstel, Germany

6 National Reference Laboratory for Mycobacteria, “Sotiria” Chest Diseases Hospital, Athens, Greece

7 Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy

8 Division de Bactériologie – Parasitologie, Laboratoire National de Santé, Luxembourg, Luxembourg

9 National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands

10 National Reference Laboratory for Mycobacteria, University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia

11 International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark

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BMC Infectious Diseases 2014, 14:62  doi:10.1186/1471-2334-14-62

Published: 6 February 2014

Abstract

Background

Since non-tuberculous mycobacteria (NTM) disease is not notifiable in most European Union (EU) and European Economic Area (EEA) countries, the epidemiological situation of the >150 NTM species is largely unknown. We aimed to collect data on the frequency of NTM detection and NTM species types in EU/EEA countries.

Methods

Officially nominated national tuberculosis reference laboratories of all EU/EEA countries were asked to provide information on: laboratory routines for detection and identification of NTM, including drug sensitivity testing (DST) methods; data on the number and type of NTM species identified; coverage and completeness of the provided data on NTM; type and number of human specimens tested for NTM; and number of specimens tested for Mycobacterium tuberculosis complex and NTM. This information was summarized and the main results are described.

Results

In total, 99 different NTM species were identified with M. avium, M. gordonae, M. xenopi , M. intracellulare, and M. fortuitum identified most frequently. Seven percent of the NTM species could not be identified. NTM was cultured from between 0.4-2.0% of the specimens (data from four countries). The laboratories use culturing methods optimised for M. tuberculosis complex. Identification is mainly carried out by a commercial line probe assay supplemented with sequencing. Most laboratories carried out DST for rapid growers and only at the explicit clinical request for slow growers.

Conclusion

It is likely that the prevalence of NTM is underestimated because diagnostic procedures are not optimized specifically for NTM and isolates may not be referred to the national reference laboratory for identification. Due to the diagnostic challenges and the need to establish the clinical relevance of NTM, we recommend that countries should concentrate detection and identification in only few laboratories.

Keywords:
Non-tuberculous mycobacteria; Mycobacterioses; Epidemiology; European Union