Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus
1 Department of Immunology, National Institute of Infectious Diseases, Shinjuku Tokyo 162-8640, Japan
2 Department of Medical Technology, School of Human Sciences, Tokyo University of Technology, 5-23-22 Nishi-Kamata, Ohta-ku Tokyo 144-8535, Japan
3 Tsuruga Institute of Biotechnology, Toyobo, Co., Ltd., Fukui 914-8550, Japan
4 National Institute of Infectious Diseases, Influenza Virus Research Center, Musashimurayama Tokyo 208-0011, Japan
5 Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
6 Pasteur Institute, National Influenza Center, Ho Chi Minh City, Vietnam
BMC Infectious Diseases 2014, 14:362 doi:10.1186/1471-2334-14-362Published: 3 July 2014
Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.
By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.
We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.
This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.