|Organisma||Target||Efficiency (%)||Linear range (fg/reaction)||Repeatability (SD of Cq)b||LOD gDNA (fg/reaction)c|
|B. mallei||BuMC||99,0||1.4·10-1 – 1.4·106||0,072||0.2|
|mau||98,7||1.4·100 – 1.4·106||0,080||4.5|
|B. pseudomallei||BuMC||96,6||1·100 - 1·107||0,085||3,7|
|psu||99,2||1·101 – 1·107||0,077||57|
a Organism from which genomic DNA was used for assessment of assay performance.
b Values represent the average from the standard deviations calculated at 6 different dilutions from 4 replicate Cq measurements.
c Values displayed represent the lowest DNA concentration at which 95% of the positive samples are detected, as calculated by using probit analysis.
Janse et al.
Janse et al. BMC Infectious Diseases 2013 13:86 doi:10.1186/1471-2334-13-86