Table 3

Assay performance
Organisma Target Efficiency (%) Linear range (fg/reaction) Repeatability (SD of Cq)b LOD gDNA (fg/reaction)c
B. mallei BuMC 99,0 1.4·10-1 – 1.4·106 0,072 0.2
mau 98,7 1.4·100 – 1.4·106 0,080 4.5
B. pseudomallei BuMC 96,6 1·100 - 1·107 0,085 3,7
psu 99,2 1·101 – 1·107 0,077 57

a Organism from which genomic DNA was used for assessment of assay performance.

b Values represent the average from the standard deviations calculated at 6 different dilutions from 4 replicate Cq measurements.

c Values displayed represent the lowest DNA concentration at which 95% of the positive samples are detected, as calculated by using probit analysis.

Janse et al.

Janse et al. BMC Infectious Diseases 2013 13:86   doi:10.1186/1471-2334-13-86

Open Data