Co-infection of human parvovirus B19 with Plasmodium falciparum contributes to malaria disease severity in Gabonese patients
- Equal contributors
1 Department of Molecular Pathology, Institute of Pathology and Neuropathology, University of Tuebingen, Tuebingen, Germany
2 Vietnam Military Medical University, Ha Dong, Ha Noi, Viet Nam
3 Tran Hung Dao Hospital, 108 Institute of Clinical Medical and Pharmaceutical Sciences, Ha Noi, Viet Nam
4 Vietnam Military Medical Bureau, Ha Noi, Viet Nam
5 Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany
6 Centre de Recherche Médicale de Lambaréné (CERMEL), Lambaréné P.B.118, Gabon
7 Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany
BMC Infectious Diseases 2013, 13:375 doi:10.1186/1471-2334-13-375Published: 15 August 2013
High seroprevalence of parvovirus B19 (B19V) coinfection with Plasmodium falciparum has been previously reported. However, the impact of B19V-infection on the clinical course of malaria is still elusive. In this study, we investigated the prevalence and clinical significance of B19V co-infection in Gabonese children with malaria.
B19V prevalence was analyzed in serum samples of 197 Gabonese children with P. falciparum malaria and 85 healthy controls using polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and direct DNA-sequencing.
B19V was detected in 29/282 (10.28%) of Gabonese children. B19V was observed more frequently in P. falciparum malaria patients (14.21%) in comparison to healthy individuals (1.17%) (P<0.001). Notably, the mild-malaria group revealed significantly lower hematocrit levels in B19V/P. falciparum co-infection than in P. falciparum mono-infection (P<0.05). Genetic analysis revealed a predominance of B19V genotype-1 (71.43%) in the studied population. However, B19V-genotype 2 was observed significantly more often in children with severe-malaria than in mild-malaria (P=0.04).
Our findings reveal that B19V-infection is frequent in Gabonese children with P. falciparum malaria and signifies a possible contribution of B19V on the clinical course of malaria in a genotype-dependent manner. B19V co-infection should be considered as a additional diagnostic measure in malaria patients with life threatening anemia.