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Open Access Research article

Characterisation and analysis of thioredoxin peroxidase as a potential antigen for the serodiagnosis of sarcoptic mange in rabbits by dot-ELISA

Runhui Zhang1, Wanpeng Zheng1, Xuhang Wu1, Quwu Jise1, Yongjun Ren2, Xiang Nong1, Xiaobin Gu1, Shuxian Wang1, Xuerong Peng3, Songjia Lai4 and Guangyou Yang1*

Author Affiliations

1 Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China

2 Sichuan Academy of Animal Husbandry Sciences, Chengdu 610066, China

3 Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an 625014, China

4 College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China

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BMC Infectious Diseases 2013, 13:336  doi:10.1186/1471-2334-13-336

Published: 22 July 2013

Abstract

Background

Scabies caused by Sarcoptes scabiei is a widespread but a neglected tropical zoonosis. In this study, we characterised a S. scabiei thioredoxin peroxidase (SsTPx) and evaluated a recombinant SsTPx as a diagnostic antigen in rabbits.

Methods

The open reading frame of the gene encoding SsTPx-2 was amplified and the recombinant protein was expressed in Escherichia coli cells and purified. SsTPx was localized in mite tissue by immunolocalisation using the purified recombinant protein. Serodiagnosis assays were carried out in 203 New Zealand White rabbit serum samples by dot-ELISA.

Result

The open reading frame (489 bp) of the gene encodes an 18.11 kDa protein, which showed highly homology to that of Psoroptes cuniculi (98.77% identity) and belongs to the 2-Cys family of peroxiredoxins. SsTPx was mainly distributed in muscle tissues of mites, integument of the epidermis and the anterior end of S. scabiei. Although SsTPx cross-reactivity with psoroptic mites was observed, the SsTPx dot-ELISA showed excellent diagnostic ability, with 95.3% sensitivity and 93.8% specificity in mange-infected and uninfected groups.

Conclusions

This study showed that the purified SsTPx is a highly sensitive antigen for the diagnosis of mange infection by dot-ELISA. This technique is a rapid and convenient method that can be used worldwide for the clinical diagnosis of sarcoptic mange in rabbits, and is especially useful in developing regions.

Keywords:
Sarcoptes scabiei; Thioredoxin peroxidase; Immunolocalisation; Dot-ELISA; Serodiagnosis