Table 1

Real-time PCR primers and probes[16-18]
Oligonuclotide Sequence Final conc. (nM)
ctrA forward 5′-TGTGTTCCGCTATACGCCATT-3′ 300
ctrA reverse 5′-GCCATATTCACACGATATACC-3′ 900
ctrA probe 5′-FAM-AACCTTGAGCAA″T″CCATTTATCCTGACGTTCT-3′-SpC6* 100
bexA forward 5′-TGCGGTAGTGTTAGAAAATGGTATTATG-3′ 600
bexA reverse 5′-GGACAAACATCACAAGCGGTTA-3′ 600
bexA probe 5′-FAM-ACAAAGCGTATCAA″T″ACTACAACGAGACGCAAAAA-3′-SpC6* 100
lytA forward 5′-ACGCAATCTAGCAGATGAAGCA-3′ 200
lytA reverse 5′-TCGTGCGTTTTAATTCCAGCT 200
lytA probe 5′-FAM-TGCCGAAAACGCTTGATACAGGGAG-3′-BHQ1 200

* The quencher BHQ-1 is attached to the internal thymine residue “T”.

Wu et al.

Wu et al. BMC Infectious Diseases 2013 13:26   doi:10.1186/1471-2334-13-26

Open Data