Independent individual validations of randomly selected differentially regulated miRNAs from Taqman array platform. qRT-PCR was used to validate the expression of selected miRNAs from the high throughput results (derived by StatMiner) using a specific primer and probe for each miRNA. (A) miRNAs selected from Control versus LVL comparison; (B) miRNAs selected from control versus HVL comparison. Fold increase/decrease was calculated based on normalization to U6. Average fold change for each miRNA represents fold change obtained from independent donors (N = 5 per group).
Duskova et al. BMC Infectious Diseases 2013 13:250 doi:10.1186/1471-2334-13-250