Table 3

IC50 values for TgCPB and TgCPL peptide binding to MHC class II molecules obtained using the immune epitope database
MHC II Allele1 Start-Stop2 Sequence3 Percentile Rank4
TgCPB
HLA-DRB1*01:01 10–24 LGTPLPFAALAAILL 0.01
HLA-DRB1*01:01 71–85 ESLFDSIVAEQVVAT 6.21
HLA-DRB1*01:01 228–242 LRFRYLSLKDAKKLM 4.99
HLA-DRB1*01:01 320–334 SQGKRLMPLSAQHTT 2.74
HLA-DRB1*01:01 470–484 SGVYKHVSGLPVGGH 1.58
H2-IAb 8–22 RVLGTPLPFAALAAI 3.83
H2-IAb 59–73 DVLNAFVSPESVESL 3.51
H2-IAb 187–201 VFWESRPASSNAAVA 0.91
H2-IAb 300–314 CGSCWAFASTEAFND 4.46
H2-IAb 549–563 LPGQRAAGARAGAHA 3.19
H2-IAd 15–29 PFAALAAILLLGCMY 1.88
H2-IAd 191–205 SRPASSNAAVALIKK 4.34
H2-IAd 227–241 SLRFRYLSLKDAKKL 1.21
H2-IAd 320–334 SQGKRLMPLSAQHTT 3.19
H2-IAd 428–442 QDTHKATSAYSLRSR 2.52
H2-IEd 182–196 ETGEDVFWESRPASS 17.89
H2-IEd 226–240 VSLRFRYLSLKDAKK 13.43
H2-IEd 351–365 QPGMAWRWFERKGVV 4.77
H2-IEd 399–413 DATLVPRKTPKCRKD 16.82
H2-IEd 432–446 KATSAYSLRSRDDVK 9.89
TgCPL
HLA-DRB1*01:01 60–74 RAWIALVAAAVSLLV 0.04
HLA-DRB1*01:01 46–60 YFWKKFLRQRNFTAR 6.00
HLA-DRB1*01:01 117–131 FSSFQAMYAKSYATE 2.74
HLA-DRB1*01:01 136–150 RRYAIFKNNLVYIHT 3.24
HLA-DRB1*01:01 178–192 LGFKKSRNLKSHHLG 5.20
H2-IAb 27–41 RRGVRAGRPSPPFVV 1.49
H2-IAb 59–73 ARAWIALVAAAVSLL 3.37
H2-IAb 85–99 EDDRAVFPPSPVEDH 3.42
H2-IAb 116–130 AFSSFQAMYAKSYAT 5.68
H2-IAb 226–240 CGSCWAFSTTGALEG 2.49
H2-IAd 22–36 GELHQRRGVRAGRPS 6.17
H2-IAd 57–71 FTARAWIALVAAAVS 0.14
H2-IAd 117–131 FSSFQAMYAKSYATE 2.63
H2-IAd 318–332 DVPRRSEAAMKAALA 0.01
H2-IAd 395–409 RDGYMYMAMHKGEEG 1.82
H2-IEd 42–56 TTRTYFWKKFLRQRN 7.05
H2-IEd 174–188 RRKYLGFKKSRNLKS 13.03
H2-IEd 202–216 PSELPAGVDWRSRGC 28.28
H2-IEd 309–323 KVVKILGFKDVPRRS 21.70
H2-IEd 376–390 KESKKDFWIMKNSWG 24.70

1 H2-IAb, H2-IAd and H2-IEd alleles are mouse MHC class II molecules; the HLA-DRB1*01:01 allele is a human MHC class II molecule.

2 We chose 15 amino acids for analysis each time.

3 TgCPB or TgCPL sequences.

4 Low percentile = high binding.

Zhao et al.

Zhao et al. BMC Infectious Diseases 2013 13:207   doi:10.1186/1471-2334-13-207

Open Data