Neuraminidase A (H1N1) expression levels in clinical samples. (a) Duplex RT-PCR of A (H1N1) Neuraminidase (NA) and β2-microglobulin (β2-m) in the eigth A (H1N1)-positive samples (P01-P08). In all experiments, two negative Influenza-like illness patients [Neg ILI (−) 1, Neg ILI (−) 2] and positive [Pos ILI (+); H1N1 Puerto Rico/Puerto Rico/IvPR8/Puerto Rico; 98% homology] sample were included as controls. In addition, HeLa cervical cell line and a negative control with no sample (no cDNA) were included [HeLa and Neg (no cDNA), respectively]. RNA preparations, obtained from nasopharyngeal swabs of A (H1N1) positive cases were assayed using NA or β2-m specific primers (729 and 100 bp amplification product, respectively). MWM: Molecular weight marker. (b) Neuraminidase A (H1N1) expression levels determined in the same patient’s samples by quantitative RTqPCR. Viral load results are expressed as Relative Units.
Ocadiz-Delgado et al. BMC Infectious Diseases 2013 13:20 doi:10.1186/1471-2334-13-20