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Open Access Research article

Inactivation of an enterovirus by airborne disinfectants

Thomas Thevenin, Pierre-Emmanuel Lobert and Didier Hober*

Author Affiliations

Laboratory of Virology/EA3610, University Lille 2, Faculty of Medecine, CHRU Lille, Institut Hippocrate, 152 rue du Dr Yersin, Loos-Lez-Lille, 59120, France

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BMC Infectious Diseases 2013, 13:177  doi:10.1186/1471-2334-13-177

Published: 15 April 2013

Abstract

Background

The activity of airborne disinfectants on bacteria, fungi and spores has been reported. However, the issue of the virucidal effect of disinfectants spread by fogging has not been studied thoroughly.

Methods

A procedure has been developed to determine the virucidal activity of peracetic acid-based airborne disinfectants on a resistant non-enveloped virus poliovirus type 1. This virus was laid on a stainless carrier. The products were spread into the room by hot fogging at 55°C for 30 minutes at a concentration of 7.5 mL.m-3. Poliovirus inoculum, supplemented with 5%, heat inactivated non fat dry organic milk, were applied into the middle of the stainless steel disc and were dried under the air flow of a class II biological safety cabinet at room temperature. The Viral preparations were recovered by using flocked swabs and were titered on Vero cells using the classical Spearman-Kärber CPE reading method, the results were expressed as TCID50.ml-1.

Results

The infectious titer of dried poliovirus inocula was kept at 105 TCID50.mL-1 up to 150 minutes at room temperature. Dried inocula exposed to airborne peracetic acid containing disinfectants were recovered at 60 and 120 minutes post-exposition and suspended in culture medium again. The cytotoxicity of disinfectant containing medium was eliminated through gel filtration columns. A 4 log reduction of infectious titer of dried poliovirus inocula exposed to peracetic-based airborne disinfectant was obtained.

Conclusion

This study demonstrates that the virucidal activity of airborne disinfectants can be tested on dried poliovirus.