Table 4 |
||||
| Comparison of the LAMP assay with nested PCR assay for the detection of the spotted fever rickettsiae group in clinical samplesa | ||||
| No. of cases | Diagnosis type | LAMP | Nested PCR | Real-time PCR |
| 1 | gltA gene amplified | Pos. | Neg. | Pos. |
| 2 | gltA gene amplified | Pos. | Neg. | Pos. |
| 3 | 4-fold IgG titer increase | Pos. | Neg. | Neg. |
| 4 | Culture confirmed and gltA gene amplified | Pos. | Neg. | Pos. |
| 5 | 4-fold IgG titer increase | Pos. | Neg. | Neg. |
| 6 | 4-fold IgG titer increase and gltA gene amplified | Pos. | Neg. | Pos. |
| 7 | 4-fold IgG titer increase and gltA gene amplified | Pos. | Neg. | Pos. |
| 8 | Suspected case | Neg. | Neg. | Pos. |
| 9 | Culture confirmed | Neg. | Neg. | Neg. |
| 10 | 4-fold IgG titer increase | Neg. | Neg. | Neg. |
| 11 | 4-fold IgG titer increase and gltA gene amplified | Pos. | Neg. | Pos. |
a All assays were performed in triplicate.
Pan et al. BMC Infectious Diseases 2012 12:254 doi:10.1186/1471-2334-12-254
