Leptin levels out of supernatants of the adipocytes cell culture measured by ELISA showed decreased levels of leptin after incubation with TNF alpha for 6 and 24 hours (p < 0.05). Increasing levels of leptin were observed after 6 hours of incubation according to the applied dosages of DAA (50 ng/ml and 5 μg/ml). Significances (n.s. = not significant) of t-tests with Holm-Bonferroni adjustments for multiple comparisons are indicated. Only the relevant in advance specified group comparisons were analysed. Data are presented as mean with standard error of mean (SEM).
Behnes et al. BMC Infectious Diseases 2012 12:217 doi:10.1186/1471-2334-12-217