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Open Access Research article

Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis

Ernst Kristian Rødland1278*, Thor Ueland137, Stine Bjørnsen1, Ellen Lund Sagen1, Christen Peder Dahl1, Anne Naalsund4, Tom Eirik Mollnes57, Frank R Brosstad17, Fredrik Müller67, Pål Aukrust127 and Stig S Frøland127

Author Affiliations

1 Research Institute for Internal Medicine, University of Oslo, Oslo, Norway

2 Section of Clinical Immunology and Infectious Diseases, University of Oslo, Oslo, Norway

3 Department of Endocrinology, University of Oslo, Oslo, Norway

4 Department of Respiratory Medicine, University of Oslo, Oslo, Norway

5 Institute of immunology, University of Oslo, Oslo, Norway

6 Department of Microbiology, Rikshospitalet, Oslo University Hospital, University of Oslo, Oslo, Norway

7 Faculty of Medicine, University of Oslo, Oslo, Norway

8 Research Institute for Internal Medicine, Rikshospitalet, Oslo University Hospital, N-0027, Oslo, Norway

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BMC Infectious Diseases 2012, 12:144  doi:10.1186/1471-2334-12-144

Published: 25 June 2012

Abstract

Background

The purpose of this study was to investigate mediators of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis (CNPA), a locally, destructive process of the lung due to invasion by Aspergillus species.

Methods

Measurements of selected biomarkers in 10 patients with CNPA and 19 healthy, matched controls were performed with enzyme-linked immunosorbent assay (ELISA) and multiplex methodology. The gene expressions of relevant biomarkers were analyzed with real-time quantitative RT-PCR.

Results

Increased concentrations of circulating mediators of inflammation interleukin (IL)-6, IL-8, RANTES, TNF-α, ICAM-1 and mediators involved in endothelial activation and thrombosis (vWF, TF and PAI-1) were observed in patients with CNPA. The concentration of the anti-inflammatory cytokine IL-10 was increased both in plasma and in PBMC in the patient population. The gene expression of CD40L was decreased in PBMC from the patient group, accompanied by decreased concentrations of soluble (s) CD40L in the circulation.

Conclusions

The proinflammatory response against Aspergillus may be counteracted by reduced CD40L and sCD40L, as well as increased IL-10, which may compromise the immune response against Aspergillus in patients with CNPA.

Keywords:
CNPA; Inflammation; Haemostasis; Biomarkers