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Phylogenetic and recombination analysis of human bocavirus 2

Weixia Cheng12, Jinan Chen4, Ziqian Xu1, Jiemei Yu1, Canping Huang1, Miao Jin1, Huiying Li1, Ming Zhang5, Yu Jin23* and Zhao-jun Duan1*

Author Affiliations

1 State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China

2 Medical School of Nanjing University, Nanjing Children's Hospital, Nanjing 210093, PR China

3 School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, PR China

4 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China

5 Dept. of Epidemiology and Biostatistics and Faculty of Infectious Diseases, University of Georgia, Athens, GA 30602 USA

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BMC Infectious Diseases 2011, 11:50  doi:10.1186/1471-2334-11-50

Published: 24 February 2011



Human bocavirus 2(HBoV2) and other human bocavirus species (HBoV, HBoV3, and HBoV4) have been discovered recently. But the precise phylogenetic relationships among these viruses are not clear yet.


We collected 632 diarrhea and 162 healthy children in Lanzhou, China. Using PCR, Human bocavirus (HBoV), HBoV2, HBoV3 and HBoV4 were screened. The partial genes of NS, NP1 and VP, and two nearly complete sequences of HBoV2 were obtained.


Phylogenetic analysis showed the different genes of HBoV2 strain were homogenous with different reference strains. HBoV3 may be a recombinant derived from HBoV and HBoV4. We also observed that the VP1 and VP2 region of HBoV3 is as similar to HBoV2 as to HBoV4.


A single genetic lineage of HBoV2 is circulating in children with and without gastroenteritis in Lanzhou, China. Current evidence in this study was not enough to support recombination between HBoV2 strains, and HBoV3 may be a recombinant between HBoV and the common ancestor of HBoV2 and HBoV4.