Table 5

Interference of background human DNA in quantitation of HPV-16 DNA with HPV-16 E6 and HPV-16 E2 real-time PCR assays.

No. of copies of human DNA

Quantitation of 1000 copies of episomal HPV-16 DNA with real-time PCR assays


HPV-16 E2

HPV-16 E6


0

933 ± 13

1150 ± 39


8 × 103

1080 ± 35

1114 ± 7


1 × 104

1097 ± 38

1179 ± 148


2 × 104

1026 ± 149

1109 ± 59


4 × 104

1125 ± 15

1015 ± 46


6 × 104

1173 ± 10

820 ± 30


8 × 104

1151 ± 100

726 ± 19


1 × 105

1082 ± 82

787 ± 32


2 × 105

1071 ± 38

562 ± 20


1000 copies of HPV-16 DNA were amplified in a background of various copies of human DNA and detected in real-time PCR assays for quantitation of HPV-16 E6 and HPV-16 E2. Results are means ± 1 standard deviation of duplicate values. Repeat experiments gave similar results.

Ramanakumar et al. BMC Infectious Diseases 2010 10:326   doi:10.1186/1471-2334-10-326

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