Table 3

Detection limit and Ct value comparison of PCR amplification on nucleic acid extractions from inoculated blood, inoculated PBS and purified plasmid DNA

Amplification targets and Ct value

S. Typhi

S. Paratyphi A

Equivalent cfu/ml

PBS

Blood

Plasmid

PBS

Blood

Plasmid

1 × 10³

Ct value

21.27

24.78

27.77

31.16

-

1 × 10⁴

Ct value

21.42

24.66

28.28

31.84

-

1 × 10⁴

CV (%)

0.41

0.7

0.67

0.86

1.34

1 × 10⁶

CV (%)

1.34

1.34

0.86

1.25

1.83

Nga et al. BMC Infectious Diseases 2010 10:125   doi:10.1186/1471-2334-10-125