Table 1 |
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|
Primers and conditions for the RT-PCR experiments performed in this study. |
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|
Gene |
Sequence (5'→3') |
T (°C) |
Product (bp) |
|
|
|||
|
IL-2 |
GCAACTCCTGTCTTGCATTG AATGTGAGCATCCTGGTGAG |
59 |
173 |
|
IFN-γ |
AGCTCTGCATCGTTTTGGGTTC CAAATATTGCAGGCAGGACAACC |
64 |
492 |
|
IL-4 |
CTGTGCTCCGGCAGTTCTAC ACGTACTCTGGTTGGCTTCC |
58 |
176 |
|
IL-5 |
GCTTCTGCATTTGAGTTTGCTAGCT TGGCCGTCAATGTATTTCTTTATTAAG |
59 |
291 |
|
IL-10 |
ACCCAGTCTGAGAACAGCTGC GTTCACATGCGCCTTGATGTCT |
61 |
260 |
|
β2m |
CCCCCACTGAAAAAGATGAG TCACTCAATCCAAATGCGGC |
56 |
150 |
|
|
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|
Panitsas and Mouzaki BMC Blood Disorders 2004 4:4 doi:10.1186/1471-2326-4-4 |
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