Table 1

ZFN targeting and cleaving within the hemoglobin, beta (βA)-gene
Zinc Finger Nuclease (ZFN) Left Fn; triplet- α-Helix Right Fn; triplet- α-Helix
-targeting HBB (βA)-DNA at 5′ Sites
ZFN-unknown-SP-5-1
5′_82 cCGTTACTGCCCTGTGGGGCAAGGt 106_′3 F1; RNITLVR; (ACG) F1; RNEHLKV; (AGG)
5′_82 gGCAATGACGGGACACCCCGTTCCa 106_′3 F2; QRSSLVR; (GTA) F2; QSTTLKR; (GCA)
F3; QDNTLRR; (GCA) F3; RTEHLAR; (GGG)
-targeting HBB (βA)-DNA at 3′ Sites
ZFN-unknown-SP-7-1
5′_1333 cTTCCTCCCACAGCTCCTGGGCAACGt 1359_′3 F1; QASNLLR; (GAA) F1; RSQTLAQ; (ACG)
5′_1333 gAAGGAGGGTGTCGAGGACCCGTTGCa 1359_′3 F2; RQDNLGR; (GAG) F2; QSTTLKR; (GCA)
F3; RMDHLAG; (TGG) F3; RSDHLSL; (TGG)
ZFN-unknown-SP-6-1
5′_1334 tTCCTCCCACAGCTCCTGGGCAACGt 1359_′3 F1; RTDRLIR; (GGA) F1; RSQTLAQ; (ACG)
5′_1334 aAGGAGGGTGTCGAGGACCCGTTGCa 1359_′3 F2; QSAHLKR; (GGA) F2; QSTTLKR; (GCA)
F3; RNTALQH; (GTG) F3; RSDHLSL; (TGG)

Wayengera

Wayengera BMC Blood Disorders 2012 12:5   doi:10.1186/1471-2326-12-5

Open Data