Table 1

The primer sequences, exons amplified and the restriction enzymes used in the PCR/RFLP procedures to detect five G6PD deficient mutations and the silent one [20-23]

Variant

Primers

Exon amplified

Restriction Enzyme


Mediterranean

(563 C→T)

5'GGT GAG GCT CCT GAG TAC CA 3'

VI

MboII

5'AGC TGT GAT CCT CAC TCC CC 3'


Chatham

(1003 G→A)

5'CAA GGA GCC CAT TCT CTC CCT T 3'

IX

BstXI

5'TTC TCC ACA TAG AGG AGG ACG GCT GCC AAA GT3'


A-

(202 G→A)

5'CGT GTC CCC AGC CAC TTC TA 3'

III-V

NlaIII

5' CAC GCT CAT AGA GTG GTG GG 3'


Cosenza

(1376 G→C)

5'GCA GCC AGT GGG ATC AGC AAG 3'

5' GGC AAG GAG GGT GGC CGT GG 3'

XI-XIII

Bsu361


Aures

(143 T→C)

5' CAG CCA CTT CTA ACC ACA CAC Ct 3'

5' CCG AAG TTG GCC ATG CTG GG 3'

III

Mbo I


Silent

(1311 C→T)

5' TGT TCT TTC AAC CCC GAG GAG T 3'

5' AAG ACG TCC AGG ATG AGG TGA TC 3'

Part X-XI

Bcl I


Al-Allawi et al. BMC Blood Disorders 2010 10:6   doi:10.1186/1471-2326-10-6

Open Data